您好，欢迎访问吉林省农业科学院 机构知识库！
吉林省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

PROTEOMICS ANALYSIS OF OVEREXPRESSED PLASMA PROTEINS IN RESPONSE TO COLD ACCLIMATION IN Ostrinia furnacalis

文献类型： 外文期刊

作者： Shang, Qingli 1 ; Pan, Yiou 1 ; Peng, Tianfei 1 ; Yang, Shuang 1 ; Lu, Xin 2 ; Wang, Zhenying 3 ; Xi, Jinghui 1 ;

作者机构： 1.Jilin Univ, Coll Plant Sci, Changchun 130062, Peoples R China

2.Jilin Acad Agr Sci, Changchun, Peoples R China

3.Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, Beijing 100193, Peoples R China

关键词： Ostrinia furnacalis;plasma proteins;cold stress;proteomics

期刊名称：ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：1.698； 五年影响因子：1.758 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Many insects in temperate regions overwinter in diapause. In these insects, one of the metabolic adaptations to cold stress is the synthesis of responsive proteins. Using proteomic analysis, an investigation aimed to a better understanding of the molecular adaptation mechanisms to cold stress was carried out in Ostrinia furnacalis larva. Proteins were extracted from the larval hemolymph collected from both control and overwintering larva. By polyethylene glycol precipitation, approximately 560 protein spots were separated and visualized on two-dimensional (2D) gels after silver staining. Eighteen protein spots were found to be upregulated in overwinter larval plasma in different patterns. As an initial work, 13 of these proteins were identified using MALDI TOF/TOF MS. The differentially overexpressed proteins include heat shock 70 kDa cognate protein, small heat shock protein (sHSP), putative aliphatic nitrilase, arginine kinase, phosphoglyceromutase, triosephosphateisomerase, and glutathione transferase. Alterations in the levels of these proteins were further confirmed by qPCR. This study is the first analysis of differentially expressed plasma proteins in O. furnacalis diapause larvae under extremely low temperature conditions and gives new insights into the acclimation mechanisms responsive to cold stress. Our results also support the idea that energy metabolism, alanine and proline metabolism, and antioxidative reaction act in the cold acclimation of O. furnacalis diapause larvae. (C) 2015 Wiley Periodicals, Inc.

  • 相关文献

[1]Effects of Bacillus thuringiensis toxin Cry1Ac and Beauveria bassiana on Asiatic corn borer (Lepidoptera : Crambidae). Ma, Xiao-Mu,Liu, Xiao-Xia,Ning, Xia,Zhang, Bo,Han, Fei,Guan, Xiu-Min,Zhang, Qing-Wen,Tan, Yun-Feng. 2008

[2]Seasonal and geographical variation in diapause and cold hardiness of the Asian corn borer, Ostrinia furnacalis. Zhang, Hong-Gang,Wang, Zhen-Ying,He, Kang-Lai,Xie, Hai-Cui,Cai, Wan-Zhi,Li, Dun-Song,Mason, Charles E.,Lu, Xin. 2015

[3]Mass rearing and release of Trichogramma for biological control of insect pests of corn in China. He, Kang-Lai,Zhang, Fan,Lu, Xin,Babendreier, Dirk. 2014

[4]Do second generation Asia corn borer (Lepidoptera: Crambidae) immigrate to corn fields from alternate habitats?. Chen, Ri-Zhao,Li, Lian-Bing,Li, Peng-Pei,Klein, Michael G.,Li, Qi-Yun,Sheng, Cheng-Fa. 2015

[5]Proteomics Dissection of Cold Responsive Proteins Based on PEG Fractionation in Arabidopsis. Wang Shang,Xi Jinghui,Li Shanyu,Liu Xiangguo,Hao Dongyun. 2014

[6]A systematic proteomic analysis of NaCl-stressed germinating maize seeds. Meng, Ling-Bo,Meng, Ling-Bo,Chen, Yi-Bo,Wang, Yue-Feng,Wang, Bai-Chen,Lu, Tian-Cong,Lu, Tian-Cong,Qian, Chun-Rong,Yu, Yang,Ge, Xuan-Liang,Li, Xiao-Hui.

[7]Quantitative Proteomic Studies on TNBC in Premenopausal Patients. Liang Jin-long,Li Si-jie,Li Wan-feng,Fan Zhi-min,Liang Jin-long,Liu Xiang-guo,Hao Dong-yun. 2013

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：吉林省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台