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Pedobacter nanyangensis sp nov., isolated from herbicide-contaminated soil

文献类型: 外文期刊

作者: Zhang, Hao 1 ; Zhang, Jing 2 ; Song, Man 1 ; Cheng, Ming-gen 1 ; Wu, Ya-dong 1 ; Guo, Su-hui 1 ; Li, Qiang 1 ; Hong, Qing 1 ;

作者机构: 1.Nanjing Agr Univ, Coll Life Sci, Key Lab Microbiol Agr Environm, Minist Agr, Nanjing 210095, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Agr Resource & Environm, Nanjing 210014, Jian

期刊名称:INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ( 影响因子:2.747; 五年影响因子:2.889 )

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收录情况: SCI

摘要: A Gram-stain-negative, strictly aerobic, non-spore-forming, motile, rod-shdped bacterium, designated Q-4(T), was isolated from a herbicide-contaminated soil sample in Nanyang, Henan province, China. Strain Q-4(T) grew optimally in the LB medium without NaCl supplement at a pH range of 6.0-7.0 and a temperature of 30 degrees C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Q-4(T) was most closely related to 'Pedobacter zeaxanthinifaciens' TDMA-5 (97.4 % 16S rRNA gene sequence similarity), followed by Pedobacter xixiisoli S27(T) (95.8 %). The genomic DNA G+C content of strain Q-4(T) was 41.8 mol%. MK-7 was the major respiratory quinone. Phosphatidylethanolamine and phosphoaminolipid were the major polar lipids. The major fatty acids were iso-C-15:0, iso-C-17 :0 3-OH, C-16 :1 omega 6c and/or C-16 : 1 omega 7c (summed feature 3) and C-16 : 1 omega 7c/C-16 : 1 omega 6c (summed feature 3). Strain Q-41 showed low DNA DNA relatedness with 'P. zeaxanthinifaciens' TDMA-5 (21.4 +/- 0.6 %). Physiological and biochemical characteristics are able to distinguish strain Q-4(T) from the most closely related species of the genus Pedobacter. On the basis of genotypic and phenotypic data, strain Q-4(T) is considered to represent a novel species of the genus Pedobacter, for which the name Pedobacter nanyangensis sp. nov. is proposed. The type strain is Q-4(T) ( = KCTC 42442(T) = ACCC 19798(T)).

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