Cytotoxic effects and apoptosis induction of enrofloxacin in hepatic cell line of grass carp (Ctenopharyngodon idellus)
文献类型: 外文期刊
作者: Liu, Bo 1 ; Cui, Yanting 2 ; Brown, Paul B. 3 ; Ge, Xianping 1 ; Xie, Jun 1 ; Xu, Pao 1 ;
作者机构: 1.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Minist Agr, Key Lab Freshwater Fisheries & Germplasm Resource, Wuxi 214081, Peoples R China
2.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 214081, Peoples R China
3.Purdue Univ, Dept Forestry & Nat Resources, W Lafayette, IN 47907 USA
关键词: Enrofloxacin;Cytotoxicity;Apoptosis;ROS;Grass carp;Hepatic cell
期刊名称:FISH & SHELLFISH IMMUNOLOGY ( 影响因子:4.581; 五年影响因子:4.851 )
ISSN:
年卷期:
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收录情况: SCI
摘要: We determined the effect of enrofloxacin on the lactate dehydrogenase (LDH) release, reactive oxygen species (ROS), superoxide dismutase (SOD), total antioxidant capacity (T-AOC), malondialdehyde (MDA), mitochondria membrane potential (Delta Psi m) and apoptosis in the hepatic cell line of grass carp (Ctenopharyngodon idellus). Cultured cells were treated with different concentrations of enrofloxacin (12.5-200 ug/mL) for 24 h. We found that the cytotoxic effect of enrofloxacin was mediated by apoptosis, and that this apoptosis occurred in a dose-dependent manner. The doses of 50,100 and 200 mu g/mL enrofloxacin increased the LDH release and MDA concentration, induced cell apoptosis and reduced the Delta Psi m compared to the control. The highest dose of 200 ug/mL enrofloxacin also significantly induced apoptosis accompanied by Delta Psi m disruption and ROS generation and significantly reduced T-AOC and increased MDA concentration compared to the control. Our results suggest that the dose of 200 ug/mL enrofloxacin exerts its cytotoxic effect and produced ROS via apoptosis by affecting the mitochondria of the hepatic cells of grass carp. (C) 2015 Elsevier Ltd. All rights reserved.
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