您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Validating the importance of two acetylcholinesterases in insecticide sensitivities by RNAi in Pardosa pseudoannulata, an important predatory enemy against several insect pests

文献类型： 外文期刊

作者： Meng, Xiangkun 1 ; Li, Chunrui 1 ; Bao, Haibo 1 ; Fang, Jichao 2 ; Liu, Zewen 1 ; Zhang, Yixi 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Minist Educ, Key Lab Integrated Management Crop Dis & Pets, Nanjing 210095, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China

关键词： Pardosa pseudoannulata;RNA interference;Acetylcholinesterase;Insecticide sensitivity

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：3.963； 五年影响因子：4.454 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The pond wolf spider (Pardosa pseudoannulata) is an important predatory enemy against several insect pests and showed relative different sensitivities to organophosphate and carbamate insecticides compared to insect pests. In our previous studies, two acetylcholinesterases were identified in P. pseudoannulata and played important roles in insecticide sensitivities. In order to understand the contributions of the two acetylcholinesterases to insecticide sensitivities, we firstly employed the RNAi technology in the spider. For a suitable microinjection RNAi method, the injection site, injection volume and interference time were optimized, which then demonstrated that the injection RNAi method was applicable in this spider. With the new RNAi method, it was revealed that both Pp-AChE1 and Pp-AChE2, encoded by genes Ppace1 and Ppace2, were the targets of organophosphate insecticides, but Pp-AChE1 would be more important In contrast the carbamate acted selectively on Pp-AChE1. The results showed that Pp-AChE1 was the major catalytic enzyme in P. pseudoannulata and the major target of organophosphate and carbamate insecticides. In a word, an RNAi method was established in the pond wolf spider, which further validated the importance of two acetylcholinesterases in insecticide sensitivities in this spider. (C) 2015 Elsevier Inc. All rights reserved.

  • 相关文献

[1]Identification of two acetylcholinesterases in Pardosa pseudoannulata and the sensitivity to insecticides. Zhang, Yixi,Shao, Ying,Jiang, Feng,Li, Jian,Liu, Zewen,Zhang, Yixi,Shao, Ying.

[2]Cloning of Two Acetylcholinesterase Genes and Analysis of Point Mutations Putatively Associated with Triazophos Resistance in Chilo auricilius (Lepidoptera: Pyralidae). Li, Xiao-Huan,Zhang, Zhi-Chun,Liu, Bao-Sheng,Fang, Ji-Chao,Li, Xiao-Huan,Fang, Ji-Chao,Huang, Shui-Jin.

[3]Biochemistry and molecular characterisation of chlorpyrifos resistance in field strains of the white-backed planthopper Sogatella furcifera (Hemiptera: Delphacidae). He, Ming,He, Peng,Ai, Zhen-Xian,Jiang, Zi-Qiong,Long, Yu-Ning,Zhang, Yue-Liang.

[4]Cross-resistance and possible mechanisms of chlorpyrifos resistance in Laodelphax striatellus (Fallen). Wang, Lihua,Zhang, Yueliang,Liu, Yanhe,Fang, Jichao,Zhang, Yueliang,Han, Zhaojun,Liu, Yanhe. 2010

[5]Overexpression of carboxylesterase-1 and mutation (F439H) of acetylcholinesterase-1 are associated with chlorpyrifos resistance in Laodelphax striatellus. Zhang, Yueliang,Li, Shuo,Guo, Hui Fang,Zi, Jinyan,Wang, Lihua,Fang, Jichao,Xu, Lu,Zi, Jinyan,He, Peng. 2013

[6]Mutation in acetylcholinesterase1 associated with triazophos resistance in rice stem borer, Chilo suppressalis (Lepidoptera: Pyralidae). Jiang, Xiaojing,Jiang, Weihua,Han, Zhaojun,Qu, Mingjing,Denholm, Ian,Fang, Jichao. 2009

[7]Ecdysone receptor isoform-B mediates soluble trehalase expression to regulate growth and development in the mirid bug, Apolygus lucorum (Meyer-Dur). Tan, Y. -A.,Xiao, L. -B.,Zhao, J.,Sun, Y.,Bai, L. -X.,Xiao, Y. -F..

[8]Knockdown of NADPH-cytochrome P450 reductase results in reduced resistance to buprofezin in the small brown planthopper, Laodelphax striatellus (fallen). Zhang, Yueliang,Wang, Yaming,Wang, Lihua,Yao, Jing,Guo, Huifang,Fang, Jichao,Wang, Yaming.

[9]Molecular and functional characterization of the ecdysone receptor isoform-A from the cotton mirid bug, Apolygus lucorum (Meyer-Dur). Tan, Yong-An,Xiao, Liu-Bin,Zhao, Jing,Sun, Yang,Bai, Li-Xin.

[10]RNA interference of E75 nuclear receptor gene suppresses transmission of rice stripe virus in Laodelphax striatellus. Fang, Ying,Lee, Seok Hee,Kim, Jong Hoon,Park, Dong Hwan,Park, Min Gu,Woo, Ra Mi,Lee, Bo Ram,Kim, Woo Jin,Je, Yeon Ho,Choi, Jae Young,Je, Yeon Ho,Li, Shuo. 2017

[11]Reduction of infectious bursal disease virus replication by shRNAs targeting the VP1 and VP2 genes driven by chicken U6 promoter. Wei Ouyang,Ma, Jin-rong,Jiang, Jie-yuan,Wang, Yong-shan,Wang, Yong-qiang,Qin, Li-ting,Wang, Xiao-mei,Fan, Hong-jie. 2013

[12]Resistance to cycloxaprid in Laodelphax striatellus is associated with altered expression of nicotinic acetylcholine receptor subunits. Zhang, Yueliang,Han, Yangchun,Yang, Qiong,Wang, Lihua,Guo, Huifang,Fang, Jichao,He, Peng,Liu, Zewen,Li, Zhong. 2018

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台