您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Molecular cloning and characterization of two novel DREB genes encoding dehydration-responsive element binding proteins in halophyte Suaeda salsa

文献类型： 外文期刊

作者： Sun, Xiao-Bo 1 ; Ma, Hong-Xiang 1 ; Jia, Xin-Ping 1 ; Chen, Yu 2 ; Ye, Xiao-Qing 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Biotechnol, Jiangsu Key Lab Bioresources Saline Soils, Prov Key Lab Agrobiol, Nanjing 210014, Jiangsu, Peoples R China

2.Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China

关键词： Suaeda salsa L.;DREB transcription factors;Abiotic stresses;Subcellular localization;Yeast one-hybrid;Quantitative real-time PCR

期刊名称：GENES & GENOMICS （ 影响因子：1.839； 五年影响因子：1.329 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The dehydration-responsive element-binding (DREB) proteins play an important role in regulating expression of stress-inducible genes under abiotic stresses. In this study, two genes encoding putative DREB proteins, named SsDREBa and SsDREBb, were cloned from halophyte Suaeda salsa L. using RACE method. The deduced SsDREBa and SsDREBb proteins contain a typical AP2/ERF domain. Multiple sequence alignments and phylogenetic analysis revealed that the two SsDREB genes of S. salsa were highly similar in AP2/ERF domains at the nucleotide and amino acid levels and belong to the A-6 subgroup of the DREB transcription factor subfamily. A subcellular localization assay showed that both SsDREBs localized to the nucleus. Yeast one-hybrid experiments testified that both proteins were able to specifically bind to the DRE sequence and activate the expression of the down-stream HIS reporter gene in yeast. Quantitative real-time PCR analysis demonstrated that under normal conditions, the expression level of SsDREBa was the most high in the roots and no SsDREBa mRNAs were detected in the stems; SsDREBb expressed at relatively higher levels in the leaves than in the roots and stems. The expression of SsDREa and SsDREBb genes in S. salsa roots and leaves was remarkably induced by high-salt and dehydration treatments, but not by cold and ABA, and exhibited stronger induction in roots and leaves, respectively. These results indicate that the SsDREBa and SsDREBb are novel stress-responsive transcription factors, which are involved in the drought and high-salt stress responses through ABA-independent pathways and could be used for production of stress-tolerant transgenic crops.

  • 相关文献

[1]Overexpression of a PIP1 Gene from Salicornia bigelovii in Tobacco Plants Improves Their Drought Tolerance. Sun, Xiaobo,Deng, Yanming,Liang, Lijian,Jia, Xinping,Xiao, Zheng,Su, Jiale.

[2]Systematic discovery and characterization of stress-related microRNA genes in Oryza sativa. Xie, Kai Bin,Zhou, Xue,Zhang, Tian Hai,Chen, Guo Xiang,Zhou, Xue,Zhang, Bao Long,Chen, Li Ming.

[3]Cloning and expression analysis of CaPIP1-1 gene in pepper (Capsicum annuum L.). Yin, Yan-Xu,Zhang, Huai-Xia,Xiao, Huai-Juan,Jin, Jing-Hao,Ji, Jiao-Jiao,Jing, Hua,Chen, Ru-Gang,Arisha, Mohamed Hamed,Gong, Zhen-Hui,Wang, Shu-Bin,Arisha, Mohamed Hamed.

[4]Genome-wide identification of the auxin response factor (ARF) gene family and expression analysis of its role associated with pistil development in Japanese apricot (Prunus mume Sieb. et Zucc). Gao, Zhihong,Huo, Ximei,Xu, Yanshuai,Shi, Ting,Ni, Zhaojun,Song, Juan,Sun, Hailong.

[5]Selection of appropriate reference genes in eggplant for quantitative gene expression studies under different experimental conditions. Zhou, Xiaohui,Liu, Jun,Zhuang, Yong.

[6]De novo characterization of the Iris lactea var. chinensis transcriptome and an analysis of genes under cadmium or lead exposure. Gu, Chun-Sun,Liu, Liang-Qin,Zhang, Yong-Xia,Wang, Zhi-Quan,Yuan, Hai-Yan,Huang, Su-Zhen,Gu, Chun-Sun,Liu, Liang-Qin,Zhang, Yong-Xia,Wang, Zhi-Quan,Yuan, Hai-Yan,Huang, Su-Zhen,Deng, Yan-Ming. 2017

[7]Genome-wide identification and expression analysis of beta-galactosidase family members during fruit softening of peach [Prunus persica (L.) Batsch]. Guo, Shaolei,Song, Juan,Zhang, Binbin,Jiang, Hang,Ma, Ruijuan,Yu, Mingliang,Guo, Shaolei,Jiang, Hang,Guo, Shaolei,Song, Juan,Zhang, Binbin,Jiang, Hang,Ma, Ruijuan,Yu, Mingliang. 2018

[8]Genome-wide characterization of the ankyrin repeats gene family under salt stress in soybean. Zhang, Dayong,Wan, Qun,He, Xiaolan,Ning, Lihua,Huang, Yihong,Xu, Zhaolong,Liu, Jia,Shao, Hongbo,Shao, Hongbo.

[9]Cloning and expression analysis of PpSUT2 encoding a sucrose transporter in pear. Tang, J.,Lin, J.,Zhang, B. L.,Wang, Z. H.,Li, X. G.,Chang, Y. H.,Tang, J.. 2014

[10]Genome-wide analysis of the TPX2 family proteins in Eucalyptus grandis. Gan, Siming,Du, Pingzhou,Yao, Yuan,Wang, Yuqi,Du, Pingzhou,Yao, Yuan,Xie, Qiaoli,Wu, Ai-Min,Du, Pingzhou,Yao, Yuan,Xie, Qiaoli,Wu, Ai-Min,Du, Pingzhou,Wang, Jinyan,Zhang, Baolong,Kumar, Manoj,Wang, Yuqi. 2016

[11]Investigation on subcellular localization of Rice stripe virus in its vector small brown planthopper by electron microscopy. Deng, Jinhua,Li, Shuo,Ji, Yinghua,Zhou, Yijun,Deng, Jinhua,Hong, Jian. 2013

[12]Characterization and subcellular localization of an RNA silencing suppressor encoded by Rice stripe tenuivirus. Xiong, Ruyi,Wu, Jianxiang,Zhou, Xueping,Zhou, Yijun. 2009

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台