您好，欢迎访问河南省农业科学院 机构知识库！
河南省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Rapid and quantitative detection of Pythium inflatum by real-time fluorescence loop-mediated isothermal amplification assay

文献类型： 外文期刊

作者： Cao, Yanyong 1 ; Li, Yongqiang 2 ; Li, Jingjing 1 ; Wang, Lifeng 1 ; Cheng, Zeqiang 1 ; Wang, Hao 1 ; Fan, Zaifeng 3 ; Li 1 ;

作者机构： 1.Henan Acad Agr Sci, Inst Cereal Crops, Zhengzhou 450002, Peoples R China

2.Beijing Agr Univ, Plant Sci & Technol Coll, Beijing 102206, Peoples R China

3.China Agr Univ, State Key Lab Agrobiotechnol, Minist Agr, Key Lab Plant Pathol, Beijing 100094, Peoples R China

4.China Agr Univ, Dept Plant Pathol, Beijing 100094, Peoples R China

关键词： Pythium inflatum;Real-time LAMP;Quantitative detection

期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.907； 五年影响因子：2.022 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Pythium inflatum is the causal agent of Pythium maize stalk rot, which is one of the most devastating diseases of maize (Zea mays L.). P. inflatum is currently a major concern in global maize production. To the best of our knowledge, no effective resistance to P. inflatum is known in maize, and no effective measures have been reported for the control of this pathogen once maize plants have been infected. Early and accurate detection of P. inflatum is essential to guide maize planting and to protect maize production. A real-time fluorescence loop-mediated isothermal amplification (RealAmp) assay was developed for the rapid quantitative detection of P. inflatum in soil. The detection limit of the RealAmp assay was approximately 0.1 pg/mu l plasmid DNA when mixed with extracted soil DNA or 10(3) spores/g of artificially infested soil. No cross-reactions with other related pathogens were observed. Results of the RealAmp assay for quantifying the genomic DNA of P. inflatum were confirmed by testing with artificially and naturally infested samples. The quantification of the soil-borne pathogen DNA of P. inflatum in naturally infested samples was not significantly different compared with classic real-time PCR (P < 0.05). Additionally, the RealAmp assay could be detected via an improved closed-tube visual detection system by adding SYBR Green I fluorescent dye to the inside of the lid prior to amplification. Consequently, the inhibitory effects of the stain on DNA amplification were avoided. Therefore, the assay could be used more conveniently in the field as a simple, rapid, and effective technique and has the potential to become an alternative tool for detecting and monitoring P. inflatum in the field.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：河南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台