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Gymnocypris przewalskii decreases cytosolic carbonic anhydrase expression to compensate for respiratory alkalosis and osmoregulation in the saline-alkaline lake Qinghai

文献类型： 外文期刊

作者： Yao, Zongli 1 ; Guo, Wenfei 1 ; Lai, Qifang 1 ; Shi, Jianquan 2 ; Zhou, Kai 1 ; Qi, Hongfang; Lin, Tingting 1 ; Li, Zi 1 ;

作者机构： 1.Chinese Acad Fisheries Sci, Engn Res Ctr Saline Alkaline Fisheries, East China Sea Fisheries Res Inst, Shanghai 20090, Peoples R China

2.Rescue Ctr Naked Carp Lake Qinghai, Xining 810016, Qinghai, Peoples R Ch

关键词： Cytosolic carbonic anhydrase;Gymnocypris przewalskii;Respiratory alkalosis;Lake Qinghai

期刊名称：JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY （ 影响因子：2.517； 五年影响因子：2.689 ）

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收录情况： SCI

摘要： Naked carp (Gymnocypris przewalskii), endemic to the saline-alkaline Lake Qinghai, have the capacity to tolerate combined high salinity and alkalinity, but migrate to spawn in freshwater rivers each year. In this study, the full-length cDNA of the cytosolic carbonic anhydrase c isoform of G. przewalskii (GpCAc) was amplified and sequenced; mRNA levels and enzyme activity of GpCAc and blood chemistry were evaluated to understand the compensatory responses as the naked carp returned to the saline-alkaline lake after spawning. We found that GpCAc had a total length of 1400 bp and encodes a peptide of 260 amino acids. Comparison of the deduced amino acid sequences and phylogenetic analysis showed that GpCAc was a member of the cytosolic carbonic anhydrase II-like c family. Cytosolic-carbonic-anhydrase-c-specific primers were used to analyze the tissue distribution of GpCAc mRNA expression. Expression of GpCAc mRNA was found in brain, gill, liver, kidney, gut, and muscle tissues, but primarily in the gill and posterior kidney; however, none was evident in red blood cells. Transferring fish from river water to lake water resulted in a respiratory alkalosis, osmolality, and ion rise in the blood, as well as significant decreases in the expression and enzyme activity of GpCAc in both the gill and kidney within 96 h. These results indicate that GpCAc may play an important role in the acclimation to both high salinity and carbonate alkalinity. Specifically, G. przewalskii decreases cytosolic carbonic anhydrase c expression to compensate for a respiratory alkalosis and to aid in osmoregulation during the transition from river to saline-alkaline lake.

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