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Combined alkali-photocatalytic stimulation enables click microbial domestication for boosted ammonia nitrogen removal

文献类型： 外文期刊

作者： Sun, Zhen 1 ; Du, Mingzhu 2 ; Yao, Zongli 1 ; Wang, Ming 2 ; Gao, Pengcheng 1 ; Liu, Nian 2 ; Liu, Qinhong 2 ; Kang, Shifei 2 ; Lai, Qifang 1 ;

作者机构： 1.Chinese Acad Fisheries Sci, East China Sea Fisheries Res Inst, Shanghai 200093, Peoples R China

2.Univ Shanghai Sci & Technol, Dept Environm Sci & Engn, Shanghai 200093, Peoples R China

3.Univ Shanghai Sci & Technol, Inst Photochem & Photofunct Mat IPPM, Shanghai 200093, Peoples R China

关键词： Microbial domestication; Saline-alkaline stress; Photocatalytic stimulation; Ammonia nitrogen removal; Microbial nitrogen conversion

期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：12.2； 五年影响因子：11.9 ）

ISSN： 0304-3894

年卷期： 2024 年 478 卷

页码：

收录情况： SCI

摘要： Microbe-driven ammonia nitrogen removal plays a crucial role in the nitrogen cycle and wastewater treatment. However, the rational methods and mechanisms for boosting nitrogen conversion through microbial domestication are still limited. Herein, a combined alkali-photocatalytic stimulation strategy was developed to activate the Halomonas shizuishanensis DWK9 for efficient ammonia nitrogen removal. The strain DWK9 selected from saline-alkaline soil in Northwestern China possessed strong resistance to stress of saline-alkaline environment and free radicals, and was abundant in nitrogen conversion genes, thus is an ideal model for advanced microbial domestication. Bacterial in the combined alkali-photocatalytic stimulation group achieved a high ammonia nitrogen conversion rate of 67.5 %, 10 times outperforming the non-stimulated and single alkali/photocatalytic stimulation control groups. Morphology analysis revealed that the bacteria in the alkali-photocatalytic stimulated group formed a favorable structure for bioelectric transfer. Remarkably, the domesticated bacteria demonstrated improved electrochemical properties, including increased current capacity and lower overpotentials and impedance. Prokaryotic transcription genetic analysis together with qPCR analysis showed upregulation of denitrification-related metabolic pathway genes. A novel FAD dependent and NAD(P)H independent energy mode has been proposed. The universality and effectiveness of the as-developed combined alkaliphotocatalytic microbial domestication strategy were further validated through indicator fish survival experiments. This work provides unprecedented degrees of freedom for the exploration of rational microbial engineering for optimized and controllable biogeochemical conversion.

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