文献类型： 外文期刊
作者： Chen, Lingyun 1 ; Cheng, Xiaofei 2 ; Cai, Jianyu 1 ; Zhan, Linlin 1 ; Wu, Xiaoxia 3 ; Liu, Qi 4 ; Wu, Xiaoyun 1 ;
作者机构： 1.Zhejiang Agr & Forestry Univ, Coll Agr & Food Sci, Linan 311300, Zhejiang, Peoples R China
2.Hangzhou Normal Univ, Coll Life & Environm Sci, Hangzhou 310036, Zhejiang, Peoples R China
3.Northeast Agr Univ, Key Lab Soybean Biol, Chinese Minist Educ, Coll Agron, Harbin 150030, Heilongjiang, Peoples R China
4.Heilongjiang Acad Agr Sci, Inst Hort Sci, Harbin 150069, Heilongjiang, Peoples R China
关键词： Artificial trans-acting siRNA;Multiple virus resistance;TAS;Cucumber mosaic virus;Turnip mosaic virus
期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）
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收录情况： SCI
摘要： Plant TAS gene encoded trans-acting siRNAs (ta-siRNAs) regulate the expression of target mRNAs by guiding their cleavage at the sequence complementary region as microRNAs. Since one TAS transcript is cleaved into multiple ta-siRNAs in a phased manner, TAS genes may be engineered to express multiple artificial ta-siRNAs (ata-siRNAs) that target multiple viruses at several distinct genomic positions. To test this hypothesis, the Arabidopsis TAS3a gene was engineered to express ata-siRNAs targeting the genome of Turnip mosaic virus (TuMV) and Cucumber mosaic virus (CMV). Transgenic Arabidopsis thaliana plants expressing these ata-siRNAs showed high level of resistance to both viruses. These results suggest that plant TAS genes can be modified to express artificial ta-siRNAs to confer multiple virus resistance and could have broad applications for future development in virus resistance strategies. (C) 2015 Elsevier B.V. All rights reserved.
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