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Collaborative trial for the validation of event-specific PCR detection methods of genetically modified papaya Huanong No.1

文献类型： 外文期刊

作者： Wei, Jiaojun 1 ; Le, Huangying 2 ; Pan, Aihu 1 ; Xu, Junfeng 3 ; Li, Feiwu 4 ; Li, Xiang 5 ; Quan, Sheng 1 ; Guo, Jinchao 1 ;

作者机构： 1.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Natl Ctr Mol Characterizat Genetically Modified O, Shanghai 200240, Peoples R China

2.Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Key Lab Syst Biomed, Minist Educ, Shanghai 200240, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Agr Qual & Standard Agroprod, Hangzhou 310021, Zhejiang, Peoples R China

4.Jinlin Acad Agr Sci, Agrobiotech Res Ctr, Changchun 130124, Peoples R China

5.Shanghai Entry Exit Inspect & Quarantine

关键词： Method validation;GM papaya Huanong No.1;Qualitative and quantitative PCR

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

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收录情况： SCI

摘要： For transferring the event-specific PCR methods of genetically modified papaya Huanong No.1 to other laboratories, we validated the previous developed PCR assays of Huanong No.1 according to the international standard organization (ISO) guidelines. A total of 11 laboratories participated and returned their test results in this trial. In qualitative PCR assay, the high specificity and limit of detection as low as 0.1% was confirmed. For the quantitative PCR assay, the limit of quantification was as low as 25 copies. The quantitative biases among ten blind samples were within the range between 0.21% and 10.04%. Furthermore, the measurement uncertainty of the quantitative PCR results was calculated within the range between 0.28% and 2.92% for these ten samples. All results demonstrated that the Huanong No.1 qualitative and quantitative PCR assays were creditable and applicable for identification and quantification of GM papaya Huanong No.1 in further routine lab analysis. (C) 2015 Elsevier Ltd. All rights reserved.

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[2]Collaborative Ring Trial of the Papaya Endogenous Reference Gene and Its Polymerase Chain Reaction Assays for Genetically Modified Organism Analysis. Wei, Jiaojun,Guo, Jinchao,Zhang, Dabing,Yang, Litao,Li, Feiwu,Li, Xiang,Xu, Junfeng,Wu, Gang.

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