您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

A novel rapid detection method for chicken adulteration based on recombinant polymerase amplification and multicomponent nuclease (MNAzyme)

文献类型： 外文期刊

作者： Ye, Huixing 1 ; Xu, Haoyi 1 ; Xu, Junfeng 1 ; Liang, Jingang 1 ; Huang, Tao 1 ; Wang, Xiaofu 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Minist Agr & Rural Affairs, State Key Lab Managing Biot & Chem Threats Qual &, Key Lab Traceabil Agr Genet Modified Organisms, Hangzhou 310021, Peoples R China

2.Ningbo Univ, Coll Food & Pharmaceut Sci, Ningbo 315211, Zhejiang, Peoples R China

3.Minist Agr & Rural Affairs Peoples Republ China, Dev Ctr Sci & Technol, Beijing 100176, Peoples R China

关键词： Recombinase polymerase amplification (RPA); Multicomponent nuclease (MNAzyme); Chicken; Adulteration

期刊名称：MICROCHEMICAL JOURNAL （ 影响因子：4.9； 五年影响因子：4.5 ）

ISSN： 0026-265X

年卷期： 2024 年 204 卷

页码：

收录情况： SCI

摘要： The identification of meat adulteration is of great significance to food safety. This work reports a novel rapid detection platform for chicken adulteration based on recombinase polymerase amplification (RPA) and multicomponent nuclease (MNAzyme). The conserved sequence target of the Cytb gene was used to design RPA primers and MNAzyme. First, the RPA amplification products were introduced into the detection system, which included MNAzyme and DNA-RNA composite probes. When the chicken-derived RPA amplification product was present in the sample, it generated an "input" signal that triggered MNAzyme's catalytic core to cleave the DNARNA complex probe, leading to a fluorescence signal. The entire procedure, from nucleic acid extraction to detection, took just 60 min, and the results could be easily interpreted using a portable fluorescent thermostatic amplifier. The RPA-MNAzyme assay detected 8 copies/mu L of chicken DNA in 60 min, which is consistent with the results of the RPA assay, demonstrating that the method is highly accurate and specific (does not cross-react with nucleic acids from other animal sources). And it could detect as low as 0.5% chicken in beef and lamb. By harnessing MNAzyme to specifically identify target genes in RPA amplification products, the platform eliminates the need for RPA primer screening and RPA probe design, reducing experimental costs.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台