A novel rapid detection method for chicken adulteration based on recombinant polymerase amplification and multicomponent nuclease (MNAzyme)
文献类型： 外文期刊
作者： Ye, Huixing 1 ; Xu, Haoyi 1 ; Xu, Junfeng 1 ; Liang, Jingang 1 ; Huang, Tao 1 ; Wang, Xiaofu 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Minist Agr & Rural Affairs, State Key Lab Managing Biot & Chem Threats Qual &, Key Lab Traceabil Agr Genet Modified Organisms, Hangzhou 310021, Peoples R China
2.Ningbo Univ, Coll Food & Pharmaceut Sci, Ningbo 315211, Zhejiang, Peoples R China
3.Minist Agr & Rural Affairs Peoples Republ China, Dev Ctr Sci & Technol, Beijing 100176, Peoples R China
关键词： Recombinase polymerase amplification (RPA); Multicomponent nuclease (MNAzyme); Chicken; Adulteration
期刊名称：MICROCHEMICAL JOURNAL （ 影响因子：4.9； 五年影响因子：4.5 ）
ISSN： 0026-265X
年卷期： 2024 年 204 卷
页码：
收录情况： SCI
摘要： The identification of meat adulteration is of great significance to food safety. This work reports a novel rapid detection platform for chicken adulteration based on recombinase polymerase amplification (RPA) and multicomponent nuclease (MNAzyme). The conserved sequence target of the Cytb gene was used to design RPA primers and MNAzyme. First, the RPA amplification products were introduced into the detection system, which included MNAzyme and DNA-RNA composite probes. When the chicken-derived RPA amplification product was present in the sample, it generated an "input" signal that triggered MNAzyme's catalytic core to cleave the DNARNA complex probe, leading to a fluorescence signal. The entire procedure, from nucleic acid extraction to detection, took just 60 min, and the results could be easily interpreted using a portable fluorescent thermostatic amplifier. The RPA-MNAzyme assay detected 8 copies/mu L of chicken DNA in 60 min, which is consistent with the results of the RPA assay, demonstrating that the method is highly accurate and specific (does not cross-react with nucleic acids from other animal sources). And it could detect as low as 0.5% chicken in beef and lamb. By harnessing MNAzyme to specifically identify target genes in RPA amplification products, the platform eliminates the need for RPA primer screening and RPA probe design, reducing experimental costs.
- 相关文献
作者其他论文 更多>>
-
A Novel Quantification Method for Gene-Edited Animal Detection Based on ddPCR
作者：Wang, Kaili;Lan, Hangzhen;Ji, Yi;Peng, Cheng;Wang, Xiaofu;Yang, Lei;Xu, Junfeng;Chen, Xiaoyun;Ji, Yi;Peng, Cheng;Wang, Xiaofu;Yang, Lei;Xu, Junfeng;Chen, Xiaoyun
关键词：gene editing; MSTN; nucleic acid detection; ddPCR
-
An Ultra-Sensitive Quarantine Pathogen On-Site Detection Based on a One-Pot Asymmetric Recombinase Polymerase Amplification and MNAzyme-Assisted Target Recycling Biosensor (OAR-MNA)
作者：Yang, Lei;Peng, Cheng;Chen, Xiaoyun;Xu, Xiaoli;Wei, Wei;Wang, Xiaofu;Xu, Junfeng;Chen, Guanwei;Yan, Jiatong;Sun, Meihao;Bo, Yongming;Fang, Xiaoxue;Wu, Jian
关键词：asymmetric RPA; bacterial fruit blotch; cucumber green mottle mosaic virus; MNAzyme; one-pot; ssDNA
-
Study on structure, properties and formation mechanism of cassava starch-faba bean protein heat-induced gel
作者：Huang, Jianyu;Li, Xiaoqiong;Kuang, Jian;Li, Jianqiang;Li, Jinjun;Huang, Jianyu;Huang, Tao;Ying, Cai
关键词：Tapioca; Faba bean protein; Heat-induced gel; Intermolecular forces; Gel characteristics
-
Construction of faba bean protein isolate delivery vector based on pH-driven technology: Formation mechanism, structural characterization, and delivery potential
作者：Huang, Jianyu;Li, Xiaoqiong;Kuang, Jian;Li, Jianqiang;Li, Jinjun;Huang, Jianyu;Huang, Tao;Zhao, Hui;Li, Hongxia;Guo, Jinbin
关键词：Active substance delivery; pH-driven; Faba bean protein isolate; Bioavailability; Intermolecular interaction
-
Development of a novel Cas13a/Cas12a-mediated 'one-pot' dual detection assay for genetically modified crops
作者：Ding, Lin;Wang, Xiaofu;Chen, Xiaoyun;Xu, Xiaoli;Wei, Wei;Yang, Lei;Ji, Yi;Xu, Junfeng;Peng, Cheng;Wu, Jian
关键词：Dual CRISPR; Genetically Modified (GM) detection; One-pot reaction; Recombinase polymerase amplification; (RPA); Multiplexed lateral flow strips
-
A Novel Allelic Variant of OsAGPL2 Influences Rice Eating and Cooking Quality
作者：Dan, Yuqing;He, Yong;Peng, Ruixiao;Tian, Zhihong;Huang, Fudeng;Li, Chunshou;Song, Jiayu;Hao, Yuanyuan;Xu, Junfeng;Xu, Junfeng
关键词：rice quality;
OsAGPL2 ; white-core endosperm mutant; superior haplotype -
The Wx/SSIIa and GS3/GW7 Alleles, Both Individually and in Combination, Can Significantly Distinguish Rice Germplasm Quality
作者：Hao, Yuanyuan;Wu, Mingming;Song, Jiayu;Han, Yifei;Li, Chunshou;Huang, Fudeng;Xu, Junfeng;Xu, Junfeng;Zhu, Ying
关键词：rice quality; Wx; SSIIa; GS3; GW7; allelic combination
