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Molecular characterization and expression pattern of dmrt1 in the immature Chinese sturgeon Acipenser sinensis

文献类型: 外文期刊

作者: Leng, X. Q. 1 ; Du, H. J. 2 ; Li, C. J. 3 ; Cao, H. 1 ;

作者机构: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

2.China Three Gorges Corp, Chinese Sturgeon Res Inst, Hubei Key Lab Three Gorges Project Conservat Fish, Yichang 443100, Peoples R China

3.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat & Utilizat, Agr Minist China, Wuhan 430223, Peoples R China

关键词: dsx- and mab-3-related transcription factor 1;sex differentiation;subcellular localization;testis

期刊名称:JOURNAL OF FISH BIOLOGY ( 影响因子:2.051; 五年影响因子:2.25 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: In this study, the cDNA of dmrt1 gene from the Chinese sturgeon Acipenser sinensis was isolated and its expression pattern was characterized in different tissues of immature A. sinensis. By real-time quantitative PCR (qrtPCR) analysis, the A. sinensis dmrt1mRNA was detected mainly in gonad and with a higher level in the testis than the ovary, especially in 3 and 4 year-old samples. This indicated that the dmrt1 expression exhibited gradual testis specificity with development. The subcellular localization analysis indicated that the Dmrt1 protein exists only in germ cells and not in somatic cells. These results suggest that A. sinensis dmrt1 might be a highly specific sex differentiation gene for testis development and spermatogenesis.

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