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Design and evaluation of a polytope construct with multiple B and T epitopes against Tembusu virus infection in ducks

文献类型： 外文期刊

作者： Han, Kaikai 1 ; Zhao, Dongmin 2 ; Liu, Yuzhuo 2 ; Huang, Xinmei 2 ; Yang, Jing 3 ; Liu, Qingtao; An, Fengjiao; Li, 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Key Lab Vet Biol Engn & Technol, Natl Ctr Engn Res Vet Bioprod, Inst Vet Med,Minist Agr, Nanjing 210014, Peoples R China

2.Jiangsu Acad Agr Sci, Key Lab Vet Biol Engn & Technol, Natl Ctr Engn Res Vet Bioprod, Inst Vet Med,Minist Agr, Nanjing 210014, Peoples R China; Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China

3.Jiangsu Acad Agr Sci, Key Lab Vet Biol Engn & Technol, Natl Ctr Engn Res Vet Bioprod, Inst Vet Med,Minist Agr, Nanjing 210014, Peoples R China; Jiangsu Coinnovat Ctr

关键词： Tembusu virus;Envelope protein;Polytope DNA vaccine;Protective immunity

期刊名称：RESEARCH IN VETERINARY SCIENCE （ 影响因子：2.534； 五年影响因子：2.382 ）

ISSN：

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收录情况： SCI

摘要： Tembusu virus (TMUV) is a newly emerging pathogenic flavivirus that is causing massive economic loss in Chinese poultry industry; until now, there is no effective vaccine or drug for its prevention. Epitope-based vaccination is a promising approach to achieve protective immunity and to avoid immunopathology. In present study, based on in silico epitope selection, we optimized and proposed a polytope DNA vaccine (pVAX1-rTEM) consisting B-cell and T cell epitopes from the TMUV envelope (E) protein. The immunogenicity and protective efficacy of constructed polytope DNA vaccine was assessed by in vitro and in vivo experiments. In in vitro assays, the expressed pVAX1-rTEM showed reactivity with Tembusu positive serum. Its protective efficacy against TMUV infection was evaluated in ducks. The results showed that pVAX1-rTEM was highly immunogenic and could elicit high titer neutralizing antibodies and cell-mediated immune responses. These results indicate that pVAX1-rTEM may be a promising candidate vaccine for prevention of TMUV infection. (C) 2015 Published by Elsevier Ltd.

  • 相关文献

[1]Identification of heat shock protein A9 as a Tembusu virus binding protein on DF-1 cells. Liu, Qingtao,Huang, Xinmei,Zhao, Dongmin,Han, Kaikai,Liu, Yuzhuo,Yang, Jing,Bi, Keran,Li, Yin.

[2]Identification and molecular characterization of a novel flavivirus isolated from geese in China. Huang, Xinmei,Han, Kaikai,Zhao, Dongmin,Liu, Yuzhuo,Zhang, Jingfeng,Niu, Huimin,Li, Yin,Zhang, Kangning,Zhu, Jiangning,Wu, Dongming,Gao, Lei.

[3]Quantitative Proteomic Analysis of Duck Ovarian Follicles Infected with Duck Tembusu Virus by Label-Free LC-MS. Han, Kaikai,Zhao, Dongmin,Liu, Yuzhuo,Liu, Qingtao,Huang, Xinmei,Yang, Jing,An, Fengjiao,Lin, Yin,Han, Kaikai,Zhao, Dongmin,Liu, Yuzhuo,Liu, Qingtao,Huang, Xinmei,Yang, Jing,An, Fengjiao,Lin, Yin. 2016

[4]Generation and characterization of a monoclonal antibody against duck Tembusu virus envelope protein. Liu, Y..

[5]Domain I and II from newly emerging goose tembusu virus envelope protein functions as a dominant-negative inhibitor of virus infectivity. Zhao, Dongmin,Huang, Xinmei,Liu, Yuzhuo,Han, Kaikai,Zhang, Jingfeng,Yang, Jing,Xie, Xingxing,Li, Yin.

[6]Protective immune response against newly emerging goose tembusu virus infection induced by immunization with a recombinant envelope protein. Zhao, D.,Huang, X.,Han, K.,Liu, Y.,Yang, J.,Liu, Q.,An, F.,Li, Y..

[7]Poly (D,L-lactide-co-glycolide) nanoparticle-entrapped vaccine induces a protective immune response against porcine epidemic diarrhea virus infection in piglets. Li, Bin,Yu, Zhengyu,Sun, Bing,Xu, Xiangwei,Fan, Baochao,Guo, Rongli,He, Kongwang,Du, Luping,Yuan, Wanzhe. 2017

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