A powerless on-the-spot detection protocol for transgenic crops within 30 min, from leaf sampling up to results
文献类型： 外文期刊
作者： Wang, Liu 1 ; Wang, Rui 1 ; Yu, Yonghua 1 ; Zhang, Fang 1 ; Wang, Xiaofu 2 ; Ying, Yibin 1 ; Wu, Jian 1 ; Xu, Junfeng 2 ;
作者机构： 1.Zhejiang Univ, Coll Biosyst Engn & Food Sci, Hangzhou 310058, Zhejiang, Peoples R China
2.Zhejiang Acad Agr Sci, Hangzhou 310021, Zhejiang, Peoples R China
关键词： Crude DNA extract;Unpowered device;Loop-mediated isothermal amplification;Visual detection;Transgenic crop detection
期刊名称：ANALYTICAL AND BIOANALYTICAL CHEMISTRY （ 影响因子：4.142； 五年影响因子：3.863 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： The requirement of power-dependent instruments or excessive operation time usually restricts current nucleic acid amplification methods from being used for detection of transgenic crops in the field. In this paper, an easy and rapid detection method which requires no electricity supply has been developed. The time-consuming process of nucleic acid purification is omitted in this method. DNA solution obtained from leaves with 0.5 M sodium hydroxide (NaOH) can be used for loop-mediated isothermal amplification (LAMP) only after simple dilution. Traditional instruments like a polymerase chain reaction (PCR) amplifier and water bath used for DNA amplification are abandoned. Three kinds of dewar flasks were tested and it turned out that the common dewar flask was the best. Combined with visual detection of LAMP amplicons by phosphate (Pi)-induced coloration reaction, the whole process of detection of transgenic crops via genetically pure material (leaf material of one plant) could be accomplished within 30 min. The feasibility of this method was also verified by analysis of practical samples.
- 相关文献
作者其他论文 更多>>
-
High-content tailoring strategy to improve the multifunctionality of functional nucleic acids
作者：Chen, Keren;Zhu, Longjiao;Zhang, Yangzi;Yu, Yongxia;Xu, Wentao;Li, Jie;Huang, Kunlun;Xu, Wentao;Wang, Xiaofu;Wei, Wei
关键词：Functional nucleic acids; Tailoring strategies; High-content; Rational design; Multifunctionality; Affinity; Aptamer
-
A Point-of-Care Nucleic Acid Quantification Method by Counting Light Spots Formed by LAMP Amplicons on a Paper Membrane
作者：Chen, Yanju;Zhu, Yuanyuan;Wu, Jian;Peng, Cheng;Wang, Xiaofu;Xu, Junfeng;Chen, Huan
关键词：nucleic acid quantification; digital detection; paper membrane; probe-based LAMP
-
Microwave-Enabled Fast Preparation of a Metal-Organic Framework Hybrid Membrane for Filtration-Enhanced Simultaneous Separation and Detection of Aflatoxin B1
作者：Ma, Tongtong;Zhang, Qi;Yuan, Lei;Li, Zhishang;Zhang, Lin;Zhang, Jie;Yan, Shugang;Ying, Yibin;Fu, Yingchun;Xu, Xiahong
关键词：metal-organic framework; hybrid membrane; fluorescence sensor; filtration; mycotoxins
-
Review on the evolution in DNA-based techniques for molecular characterization and authentication of GMOs
作者：Liang, Zehui;Lu, Yuwen;Ding, Lin;Wang, Xiaofu;Chen, Xiaoyun;Xu, Junfeng;Peng, Cheng;Tang, Min
关键词：Genetically modified organisms; Molecular characterization; Next-Generation DNA Sequencing; Nanopore sequencing; Pacbio sequencing
-
Integration of transcriptome and machine learning to identify the potential key genes and regulatory networks affecting drip loss in pork
作者：Yang, Wen;Hou, Liming;Wu, Jian;Zha, Chengwan;Wu, Wangjun;Wang, Binbin
关键词：drip loss; meat quality; machine learning; RNA-seq; single-gene GSEA; WGCNA
-
A platform for precise quantification of gene editing products based on microfluidic chip-based digital PCR
作者：Chi, Jingzheng;Ding, Lin;Chi, Jingzheng;Ding, Lin;Wang, Xiaofu;Chen, Xiaoyun;Peng, Cheng;Xu, Junfeng
关键词：
-
Hypoglycemic mechanism of Tegillarca granosa polysaccharides on type 2 diabetic mice by altering gut microbiota and regulating the PI3K-akt signaling pathwaye
作者：Jiang, Qihong;Wang, Rui;Liu, Shulai;Xiang, Xingwei;Jiang, Qihong;Wang, Rui;Liu, Shulai;Xiang, Xingwei;Jiang, Qihong;Wang, Rui;Liu, Shulai;Xiang, Xingwei;Chen, Lin;Shen, Guoxin;Chen, Yin;Deng, Shanggui
关键词：Tegillarca granosa polysaccharide; Type 2 diabetes mellitus; Glycolipid metabolism; PI3K/Akt signaling pathway
