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MRI tracking of bone marrow mesenchymal stem cells labeled with ultra-small superparamagnetic iron oxide nanoparticles in a rat model of temporal lobe epilepsy

文献类型： 外文期刊

作者： Long, Qianfa 1 ; Li, Jianying 1 ; Luo, Qiang 1 ; Hei, Yue 2 ; Wang, Kai 3 ; Tian, Ye 1 ; Yang, Junle 1 ; Lei, Hulong 4 ; Qiu 1 ;

作者机构： 1.Xi An Jiao Tong Univ, Affiliated Hosp, Coll Med, Dept Neurosurg,Xian Cent Hosp, Xian 710003, Peoples R China

2.Fourth Mil Med Univ, Xijing Hosp, Dept Neurosurg, Xian 710032, Peoples R China

3.Qingdao 401Hosp PLA, Dept Neurosurg, Qingdao 266071, Peoples R China

4.Shanghai Acad Agr Sci, Inst Anim Husb & Vet Sci, Shanghai 201403, Peoples R China

5.Univ Washington, Sch Med, Dept Radiol, Seattle, WA 98195 USA

关键词： Magnetic resonance imaging;Ultrasmall superparamagnetic iron oxide;Bone marrow mesenchymal stem cells;Temporal lobe epilepsy

期刊名称：NEUROSCIENCE LETTERS （ 影响因子：3.046； 五年影响因子：2.855 ）

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收录情况： SCI

摘要： Transplantation of bone marrow mesenchymal stem cells (BMSCs) is a promising approach for treatment of epilepsy. To our knowledge, there is little research on magnetic resonance imaging (MRI) tracking of BMSCs labeled with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles in a rat model of temporal lobe epilepsy (TLE). In this study, BMSCs were pre-labeled with USPIO nanoparticles, and then the cell apoptosis, proliferation, surface antigens, and multipotency were investigated. Lithium chloride-pilocarpine induced TLE models were administered by USPIO-labeled BMSCs (U-BMSCs), BMSCs, and saline through lateral ventricle injection as the experimental group, control I group and control II group, respectively, followed by MRI examination, electroencephalography (EEG) and Prussian blue staining. The cell experimental results showed that the labeled USPIO did not affect the biological characteristics and multiple potential of BMSCs. The U-BMSCs can be detected using MRI in vitro and in vivo, and observed in the hippocampus and adjacent parahippocampal cortical areas of the epileptic model. Moreover, electroencephalographic results showed that transplanted U-BMSCs, as well as BMSCs, were capable of reducing the number of epileptiform waves significantly (P<0.01) compared with control II group. All of these findings suggest that it is feasible to track transplanted BMSCs using MRI in a rat model of TLE, and support that USPIO labeling is a valuable tool for cell tracking in the study of seizure disorders. (C) 2015 Elsevier Ireland Ltd. All rights reserved.

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