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The kinase OsSK41/OsGSK5 negatively regulates amylose content in rice endosperm by affecting the interaction between OsEBP89 and OsBP5

文献类型： 外文期刊

作者： Hu, Zejun 1 ; Niu, Fuan 1 ; Yan, Peiwen 2 ; Wang, Kai 1 ; Zhang, Lixia 1 ; Yan, Ying 1 ; Zhu, Yu 2 ; Dong, Shiqing 2 ; Ma, Fuying 2 ; Lan, Dengyong 2 ; Liu, Siwen 2 ; Xin, Xiaoyun 2 ; Wang, Ying 2 ; Yang, Jinshui 2 ; Cao, Liming 1 ; Wu, Shujun 1 ; Luo, Xiaojin 2 ;

作者机构： 1.Shanghai Acad Agr Sci, Key Lab Germplasm Innovat & Genet Improvement Grai, Minist & Prov, Minist Agr & Rural Affairs,Crop Breeding & Cultiva, Shanghai 201403, Peoples R China

2.Fudan Univ, State Key Lab Genet Engn, Shanghai 200438, Peoples R China

3.Fudan Univ, MOE Engn Res Ctr Gene Technol, Sch Life Sci, Shanghai 200438, Peoples R China

4.Jiangxi Agr Univ, MOE Key Lab Crop Physiol Ecol & Genet, Breeding Coll Agron, Nanchang 330045, Peoples R China

关键词： AP2; EREBP transcription factor; endosperm amylose content; GSK3-like family protein; MYC-like protein; OsSK41; OsGSK5; OsEBP89; OsBP5; rice grain

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：11.4； 五年影响因子：10.1 ）

ISSN： 1672-9072

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Amylose content (AC) is the main factor determining the palatability, viscosity, transparency, and digestibility of rice (Oryza sativa) grains. AC in rice grains is mainly controlled by different alleles of the Waxy (Wx) gene. The AP2/EREBP transcription factor OsEBP89 interacts with the MYC-like protein OsBP5 to synergistically regulate the expression of Wx. Here, we determined that the GLYCOGEN SYNTHASE KINASE 5 (OsGSK5, also named SHAGGY-like kinase 41 [OsSK41]) inhibits the transcriptional activation activity of OsEBP89 in rice grains during amylose biosynthesis. The loss of OsSK41 function enhanced Wx expression and increased AC in rice grains. By contrast, the loss of function of OsEBP89 reduced Wx expression and decreased AC in rice grains. OsSK41 interacts with OsEBP89 and phosphorylates four of its sites (Thr-28, Thr-30, Ser-238, and Thr-257), which makes OsEBP89 unstable and attenuates its interaction with OsBP5. Wx promoter activity was relatively weak when regulated by the phosphomimic variant OsEBP89(E)-OsBP5 but relatively strong when regulated by the nonphosphorylatable variant OsEBP89(A)-OsBP5. Therefore, OsSK41-mediated phosphorylation of OsEBP89 represents an additional layer of complexity in the regulation of amylose biosynthesis during rice grain development. In addition, our findings provide four possible sites for regulating rice grain AC via precise gene editing.

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