Preparation, antioxidant activity and protective effect of coconut testa oil extraction on oxidative damage to human serum albumin
文献类型: 外文期刊
作者: Zhang, Yufeng 1 ; Zheng, Yajun 1 ; Duan, Kejun 1 ; Gui, Qing 1 ;
作者机构: 1.Chinese Acad Trop Agr Sci, Coconut Res Inst, Wenchang 571339, Hainan, Peoples R China
2.Shaanxi Normal Univ, Coll Food Engn & Nutr Sci, Xian 710119, Shanxi, Peoples R China
关键词: Antioxidant activity;coconut testa oil;oxidative damage
期刊名称:INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY ( 影响因子:3.713; 五年影响因子:3.408 )
ISSN:
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收录情况: SCI
摘要: Extraction, physicochemical properties and fatty acid composition analysis of coconut testa oil (CTO), antioxidant activity and the protective effect on oxidative damage to human serum albumin (HSA) of coconut testa oil extract (CTOE) were investigated. Results showed that the optimal extract condition of CTO was B(3)A(2)C(2) (temperature of 60 degrees C, material-to-solvent of 1:4gmL(-1) and extraction time of 3h) with the maximum oil yield (76.83 +/- 0.53%). The obtained CTO was nondrying oil with iodine value of 14.69g per 100g, and lauric acid was the main component of 42.28%. Hydrogen peroxide scavenging activity of CTOE can reach to 49.81% at 2.5mgmL(-1), while antioxidant activity (AA) on the oxidation of linoleic acid dropped from 56.82% to 31.70% during the first 80min. CTOE could prevent HSA from oxidative damage induced by hydrogen peroxide via inhibiting the formation of protein carbonyl and increase of hydroperoxides content effectively. Total phenolic content was 68mgg(-1), and the epicatechin and catechin were 2.74 and 2.26mgg(-1) in its phenolic compositions, respectively. These all suggested CTO and CTOE might be new worthy exploiting functional sources.
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