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Enhanced tolerance and accumulation of heavy metal ions by engineered Escherichia coli expressing Pyrus calleryana phytochelatin synthase

文献类型： 外文期刊

作者： Li, Hui 1 ; Cong, Yu 2 ; Lin, Jing 1 ; Chang, Youhong 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Hort, Nanjing 210014, Peoples R China

2.Chinese Acad Sci, State Key Lab Soil & Sustainable Agr, Inst Soil Sci, Nanjing, Jiangsu, Peoples R China

关键词： Escherichia coli;Phytochelatin synthase;Heavy metal ion tolerance;Bioadsorbant

期刊名称：JOURNAL OF BASIC MICROBIOLOGY （ 影响因子：2.281； 五年影响因子：2.516 ）

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收录情况： SCI

摘要： Contamination by heavy metals is a major environmental problem worldwide and microbial bioremediation is an efficient method for removing this type of pollution. The plant enzyme phytochelatin synthase (PCS, also known as glutathione -glutamylcysteinyltransferase, EC 2.3.2.15) involved in the synthesis of phytochelatins (PCs), which are metal-binding cysteine-rich peptides, has a major role in the detoxification of heavy metals in plants. Expression of the PcPCS1 gene from the bean pear (Pyrus calleryana Dcne.) was induced after cadmium and copper treatments. However, functional analysis of this gene in vivo has not been reported. And it is or not suitable for bioremediation also needs to be assessed. In this study, we found Escherichia coli with over-expressed PcPCS1 had enhanced tolerance to cadmium, copper, sodium, and mercury. E. coli cells transformed with pPcPCS1 was found to survive in solid M9 medium containing 2.0mM Cd2+, 4.0mM Cu2+, 4.5% (w/v) Na+, or 200 mu M Hg2+. Moreover, the growth curve showed 1.5mM Cd2+, 2.5mM Cu2+, 3.5% (w/v) Na+, and 100 mu M Hg2+ had no effect on the growth of the E. coli cells transformed with pPcPCS1. Also, we found the contents of PCs and the accumulation of cadmium, copper, sodium, and mercury ions were enhanced in the recombinant E. coli strain Rosetta (DE3). These results suggested the PcPCS1 gene might be a candidate for heavy metal bioremediation via recombinant bacteria.

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