您好，欢迎访问河南省农业科学院 机构知识库！
河南省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Improvement of tolerance to lead by filamentous fungus Pleurotus ostreatus HAU-2 and its oxidative responses

文献类型： 外文期刊

作者： Zhang, Shimin 1 ; Zhang, Xiaolin 1 ; Chang, Cheng 2 ; Yuan, Zhiyong 2 ; Wang, Ting 3 ; Zhao, Yong 3 ; Yang, Xitian 3 ; Zh 1 ;

作者机构： 1.Henan Agr Univ, Coll Life Sci, Zhengzhou 450002, Peoples R China

2.China Natl Tobacco Corp, Henan Prov Corp, Zhengzhou 450018, Peoples R China

3.Henan Agr Univ, Coll Forestry, Zhengzhou 450002, Peoples R China

4.Henan Acad Agr Sci, Inst Plant Nutrient & Environm Resources, Zhengzhou 450002, Peoples R China

5.Henan Acad Agr Sci, Ind Crop Res Inst, Zhengzhou 450002, Peoples R China

关键词： Removal;Tolerance;Lead;Pleurotus ostreatus HAU-2;Filamentous fungi

期刊名称：CHEMOSPHERE （ 影响因子：7.086； 五年影响因子：6.956 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Wastewater contaminated with heavy metals is a world-wide concern. One biological treatment strategy includes filamentous fungi capable of extracellular adsorption and intracellular bioaccumulation. Here we report that an acclimated strain of filamentous fungus Pleurotus ostreatus HAU-2 can withstand Pb up to 1500 mg L-1 Pb, conditions in which the wildtype strain cannot grow. The acclimated strain grew in liquid culture under 500 mg L-1 Pb without significant abnormity in biomass and morphology, and was able to remove significant amounts of heavy metals with rate of 99.1% at 200 mg L-1 and 63.3% at 1500 mg L-1. Intracellular bioaccumulation as well as extracellular adsorption both contributed the Pb reduction. Pb induced levels of H2O2, and its concentration reached 72.9-100.9 mu mol g(-1) under 200 -1000 mg L-1 Pb. A relatively higher malonaldehyde (MDA) concentration (8.06-7.59 nmol g(-1)) was also observed at 500-1500 mg L-1 Pb, indicating that Pb exposure resulted in oxidative damage. The fungal cells also defended against the attack of reactive oxygen species by producing antioxidants. Of the three antioxidant enzymes superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), CAT was the most responsive and the maximal enzyme activity was 15.8 U mg(-1) protein. Additionally, glutathione (GSH) might also play a role (3.16-3.21 mg g(-1) protein) in detoxification under relatively low Pb concentration (100-200 mg L-1). Our findings suggested that filamentous fungus could be selected for increased tolerance to heavy metals and that CAT and GSH might be important components of this tolerance. (C) 2016 Elsevier Ltd. All rights reserved.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：河南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台