Newcastle disease virus forms inclusion bodies with features of liquid-liquid phase separation
文献类型： 外文期刊
作者： Wang, Chongyang 1 ; Duan, Liuyuan 1 ; Wang, Ting 1 ; Wang, Wenbin 2 ; Han, Yu 1 ; Hu, Ruochen 1 ; Hou, Qili 1 ; Liu, Haijin 1 ; Wang, Juan 1 ; Wang, Xinglong 1 ; Xiao, Sa 1 ; Dang, Ruyi 1 ; Wang, Junru 3 ; Zhang, Gaiping 1 ; Yang, Zengqi 1 ;
作者机构： 1.Northwest A&F Univ, Coll Vet Med, Yangling, Peoples R China
2.Shandong Acad Agr Sci, Poultry Inst, Jinan, Peoples R China
3.Northwest A&F Univ, Coll Chem & Pharm, Yangling, Peoples R China
4.Henan Acad Agr Sci, Minist Agr, Key Lab Anim Immunol, Henan Prov Key Lab Anim Immunol, Zhengzhou, Peoples R China
关键词： Newcastle disease virus; Inclusion bodies; Liquid-liquid phase separation; Nucleoprotein; Phosphoprotein
期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.3； 五年影响因子：3.5 ）
ISSN： 0378-1135
年卷期： 2023 年 284 卷
页码：
收录情况： SCI
摘要： Formation of inclusion bodies (IBs) is a hallmark of infections with negative-strand RNA viruses. Although the Newcastle disease virus (NDV) IBs had been observed in the 1950s, the characteristics of NDV IBs remained largely unknown. Here, we show that NDV infection triggers the formation of IBs that contain newly synthesized viral RNA. The structures of NDV IBs, observed by electron microscopy, were not membrane-bound. Fluorescence recovery after photobleaching a region of NDV IBs occurred rapidly, and IBs were dissolved by 1,6-hexanediol treatment, demonstrating they exhibited properties consistent with liquid-liquid phase separation (LLPS). We find the nucleoprotein (NP) and phosphoprotein (P) are sufficient to generate IB-like puncta, with the N arm domain and N core region of NP and the C terminus of P playing important roles in this process. In summary, our findings suggest that NDV forms IBs containing viral RNA, and provide insights into the formation of NDV IBs.
- 相关文献
作者其他论文 更多>>
-
Enhancing humoral and mucosal immune response of PED vaccine candidate by fusing S1 protein to nanoparticle multimerization
作者：Li, Minghui;Sun, Xueke;Wang, Siqiao;Wang, Yanan;Wang, Yue;Zhang, Gaiping;Li, Minghui;Sun, Xueke;Chen, Yilan;Wang, Siqiao;Li, Qin;Wang, Yanan;Wang, Yue;Li, Ruiqi;Zhang, Gaiping;Ding, Peiyang;Zhang, Gaiping;Ding, Peiyang;Zhang, Gaiping;Zhang, Gaiping;Zhang, Gaiping
关键词：PEDV; S1 protein; Nanoparticle; Subunit vaccine; Mucosal immunity
-
Pseudorabies virus tegument protein US2 antagonizes antiviral innate immunity by targeting cGAS-STING signaling pathway
作者：Kong, Zhengjie;Zhang, Yifeng;Guan, Kaifeng;Yao, Wanzi;Kang, Yu;Lu, Xinyi;Zhang, Gaiping;Chen, Xing;Gong, Lele;Wang, Lele;Zhang, Yuhang;Du, Yongkun;Sun, Aijun;Zhuang, Guoqing;Wan, Bo;Zhang, Gaiping;Zhao, Jianguo;Zhang, Gaiping;Zhang, Gaiping
关键词：pseudorabies virus; cGAS-STING; tegument protein US2; TRIM21; immune invasion
-
IFI16 Positively Regulates RIG-I-Mediated Type I Interferon Production in a STING-Independent Manner
作者：Shi, Xibao;Wei, Menglu;Feng, Yuwen;Yang, Yuanhao;Zhang, Xiaozhuan;Chen, Hao;Xing, Yuqi;Wang, Keqi;Wang, Wensheng;Wang, Li;Zhang, Gaiping;Wang, Aiping;Zhang, Gaiping;Zhang, Gaiping;Zhang, Gaiping;Shi, Xibao;Zhang, Gaiping
关键词：IFN-gamma-inducible protein 16; interferon gene-stimulating protein; retinoic acid-inducible gene I; type I interferon
-
A universal design of restructured dimer antigens: Development of a superior vaccine against the paramyxovirus in transgenic rice
作者：Ma, Fanshu;Zhang, Longxian;Zhang, Erqin;Zhang, Gaiping;Ma, Fanshu;Zhang, Gaiping;Ma, Fanshu;Zhang, Gaiping;Ma, Fanshu;Zhang, Gaiping;Ma, Fanshu;Xu, Qianru;Yang, Daichang;Li, Qingmei;Guo, Junqing;Li, Rui;Wang, Li;Wang, Yanan;Zhao, Xiangyue;Chai, Shujun;Yin, Heng;Li, Kunpeng;Rao, Zihe;Rao, Zihe;Rao, Zihe
关键词：structural vaccine; transgenic rice; BCR; paramyxovirus
-
Double-layered N-S1 protein nanoparticle immunization elicits robust cellular immune and broad antibody responses against SARS-CoV-2
作者：Li, Ruiqi;Chang, Zejie;Cheng, Anchun;Zhang, Gaiping;Li, Ruiqi;Zhang, Gaiping;Li, Ruiqi;Zhang, Gaiping;Li, Ruiqi;Chang, Zejie;Wang, Yanan;Li, Minghui;Chen, Yilan;Fan, Lu;Wang, Siqiao;Sun, Xueke;Liu, Siyuan;Zhang, Gaiping;Chang, Zejie;Wang, Yanan;Li, Minghui;Sun, Xueke;Liu, Siyuan;Zhang, Gaiping;Liu, Hongliang;Ding, Peiyang;Zhang, Gaiping
关键词：COVID-19; Coronavirus; SARS-CoV-2; Nanoparticle; Subunit vaccine; Variants
-
Porcine reproductive and respiratory syndrome virus triggers Golgi apparatus fragmentation-mediated autophagy to facilitate viral self-replication
作者：Zhao, Shuang-shuang;Qian, Qisheng;Zhang, Gaiping;Zhao, Shuang-shuang;Qian, Qisheng;Chen, Xin-xin;Lu, Qingxia;Xing, Guangxu;Qiao, Songlin;Li, Rui;Zhang, Gaiping;Zhang, Gaiping
关键词：autophagy; GA fragmentation; GRASP65; Nsp2; PRRSV; RAB2; ULK1
-
Phylogenetically evolutionary analysis provides insights into the genetic diversity and adaptive evolution of porcine deltacoronavirus
作者：Guo, Zhenhua;Lu, Qingxia;Jin, Qianyue;Xing, Guangxu;Zhang, Gaiping;Zhang, Gaiping;Zhang, Gaiping;Li, Peng
关键词：Porcine deltacoronavirus; Genetic diversity; Recombination; Adaptive evolution; Spike protein
