Newcastle disease virus forms inclusion bodies with features of liquid-liquid phase separation
文献类型： 外文期刊
作者： Wang, Chongyang 1 ; Duan, Liuyuan 1 ; Wang, Ting 1 ; Wang, Wenbin 2 ; Han, Yu 1 ; Hu, Ruochen 1 ; Hou, Qili 1 ; Liu, Haijin 1 ; Wang, Juan 1 ; Wang, Xinglong 1 ; Xiao, Sa 1 ; Dang, Ruyi 1 ; Wang, Junru 3 ; Zhang, Gaiping 1 ; Yang, Zengqi 1 ;
作者机构： 1.Northwest A&F Univ, Coll Vet Med, Yangling, Peoples R China
2.Shandong Acad Agr Sci, Poultry Inst, Jinan, Peoples R China
3.Northwest A&F Univ, Coll Chem & Pharm, Yangling, Peoples R China
4.Henan Acad Agr Sci, Minist Agr, Key Lab Anim Immunol, Henan Prov Key Lab Anim Immunol, Zhengzhou, Peoples R China
关键词： Newcastle disease virus; Inclusion bodies; Liquid-liquid phase separation; Nucleoprotein; Phosphoprotein
期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.3； 五年影响因子：3.5 ）
ISSN： 0378-1135
年卷期： 2023 年 284 卷
页码：
收录情况： SCI
摘要： Formation of inclusion bodies (IBs) is a hallmark of infections with negative-strand RNA viruses. Although the Newcastle disease virus (NDV) IBs had been observed in the 1950s, the characteristics of NDV IBs remained largely unknown. Here, we show that NDV infection triggers the formation of IBs that contain newly synthesized viral RNA. The structures of NDV IBs, observed by electron microscopy, were not membrane-bound. Fluorescence recovery after photobleaching a region of NDV IBs occurred rapidly, and IBs were dissolved by 1,6-hexanediol treatment, demonstrating they exhibited properties consistent with liquid-liquid phase separation (LLPS). We find the nucleoprotein (NP) and phosphoprotein (P) are sufficient to generate IB-like puncta, with the N arm domain and N core region of NP and the C terminus of P playing important roles in this process. In summary, our findings suggest that NDV forms IBs containing viral RNA, and provide insights into the formation of NDV IBs.
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