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Characterization and toxicology evaluation of chitosan nanoparticles on the embryonic development of zebrafish, Danio rerio

文献类型: 外文期刊

作者: Wang, Yanbo 1 ; Zhou, Jinru 1 ; Liu, Lin 1 ; Huang, Changjiang 2 ; Zhou, Deqing 3 ; Fu, Linglin 1 ;

作者机构: 1.Zhejiang Gongshang Univ, Lab Food Microbial Technol Zhejiang Prov, Hangzhou 310035, Zhejiang, Peoples R China

2.Wenzhou Med Univ, Inst Watershed Sci & Environm Ecol, Wenzhou 325035, Peoples R China

3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

关键词: Chitosan;Nanoparticle;Zebrafish;Toxicology

期刊名称:CARBOHYDRATE POLYMERS ( 影响因子:9.381; 五年影响因子:8.678 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: In the present study, chitosan nanoparticles were prepared, characterized and used to evaluate the embryonic toxicology on zebrafish (Danio rerio). The average particle size of chitosan nanoparticles was 84.86 nm. The increased mortality and decreased hatching rate was found in the zebrafish embryo exposure to normal chitosan particles and chitosan nanoparticles with the increased addition concentration. At 120h post-fertilization (hpf), the rate of mortality were 25.0 and 44.4% in the groups treated with chitosan nanoparticles and normal chitosan particles at 250 mg/L, respectively. At 72 hpf, the hatching rate in the groups treated with normal chitosan particles were lower (P<0.01) at 300 and 400 mg/L than those of the corresponding control groups, respectively. However, there were no significant differences between the groups treated with chitosan nanoparticles and the control groups across all the addition concentrations. More abundant typical malformation of embryos was observed in the groups treated with normal chitosan particles compared with those treated with chitosan nanoparticles. The LC50 (medium lethal concentration) of chitosan nanoparticles was 280 mg/L at 96 hpf and 270 mg/L at 120 hpf. As for normal chitosan particles, the LC50 was 257 mg/L at both 96 hpf and 120 hpf. The TC50 (medium teratogenic concentration) of the zebrafish treated with chitosan nanoparticles and normal chitosan particles were 257 mg/L and 137 mg/L, respectively. It indicated that the chitosan nanoparticles were relatively more secure compared with normal chitosan particles. (C) 2016 Elsevier Ltd. All rights reserved.

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