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The functions of the variable lipoprotein family of Mycoplasma hyorhinis in adherence to host cells

文献类型： 外文期刊

作者： Xiong, Qiyan 1 ; Wang, Jia 1 ; Ji, Yan 1 ; Ni, Bo 1 ; Zhang, Bixiong 1 ; Ma, Qinghong 1 ; Wei, Yanna 2 ; Xiao, Shaobo; Fe 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Natl Ctr Engn Res Vet Bioprod, Key Lab Vet Biol Engn & Technol, Inst Vet Med,Minist Agr, Nanjing 210014, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Natl Ctr Engn Res Vet Bioprod, Key Lab Vet Biol Engn & Technol, Inst Vet Med,Minist

关键词： Adhesion;Mycoplasma hyorhinis;Variable lipoprotein

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

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收录情况： SCI

摘要： Mycoplasma hyorhinis (M. hyorhinis) is a swine pathogen that is associated with various human cancers and contamination in cell cultures. However, no studies on the adhesion molecules of this pathogen have yet been reported. The variable lipoprotein (Vlp) family is an important surface component of M. hyorhinis. Herein, we performed several experiments to identify the function of the Vlp family in adherence to host cells. Seven recombinant Vlp (rVlp) proteins were expressed in Escherichia coli and purified by affinity chromatography. The potential role of rVlp adherence to pig kidney (PK-15) and swine tracheal epithelial (STEC) cells was then studied by indirect immunofluorescence assay and microtiter plate adherence assay. Adhesion of M. hyorhinis to PK-15 and STEC cells was specifically inhibited by the addition of a cocktail of rVIp proteins. The rVlp protein mixture was shown to bind to both PK-15 and STEC cells. The binding increased in a dose-dependent manner and could be blocked by antisera against the rVlp proteins. Most of the rVlp proteins could bind individually to both PK-15 and STEC cells except for rVlpD and rVlpF, which bound only to STEC cells. Because Vlp members vary in size among different strains and generations, they may vary in their cytoadhesion capabilities in various strains. In summary, the present results indicate that the Vlp family functions as adhesins of M. hyorhinis. (C) 2016 Published by Elsevier B.V.

  • 相关文献

[1]Characterization of the role in adherence of Mycoplasma hyorhinis variable lipoproteins containing different repeat unit copy numbers. Xiong, Qiyan,Zhang, Bixiong,Wang, Jia,Ni, Bo,Ji, Yan,Wei, Yanna,Feng, Zhixin,Liu, Maojun,Shao, Guoqing,Zhang, Bixiong,Xiao, Shaobo,Shao, Guoqing.

[2]Development of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Mycoplasma hyorhinis. Du, Gai-Mei,Liu, Mao-Jun,Wu, Yu-Zi,Xiong, Qi-Yan,Bai, Fang-Fang,Feng, Zhi-Xin,Shao, Guo-Qing,Du, Gai-Mei. 2013

[3]GFP as a marker for transient gene transfer and expression in Mycoplasma hyorhinis. Ishag, Hassan Z. A.,Liu, Maojun,Yang, Ruosong,Xiong, Qiyan,Feng, Zhixin,Shao, Guoqing,Ishag, Hassan Z. A.. 2016

[4]E-coli recA gene improves gene targeted homologous recombination in Mycoplasma hyorhinis. Ishag, Hassan Z. A.,Xiong, Qiyan,Liu, Maojun,Feng, Zhixin,Shao, Guoqing,Ishag, Hassan Z. A..

[5]Adhesion capability of first two domains at N terminus of NP_785232 protein and their interaction with a UV-absorbing component from human mucus. Du, L.,Huo, G.,Du, L.,Ju, X.,Liu, F.. 2010

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