您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

The functions of the variable lipoprotein family of Mycoplasma hyorhinis in adherence to host cells

文献类型： 外文期刊

作者： Xiong, Qiyan 1 ; Wang, Jia 1 ; Ji, Yan 1 ; Ni, Bo 1 ; Zhang, Bixiong 1 ; Ma, Qinghong 1 ; Wei, Yanna 2 ; Xiao, Shaobo; Fe 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Natl Ctr Engn Res Vet Bioprod, Key Lab Vet Biol Engn & Technol, Inst Vet Med,Minist Agr, Nanjing 210014, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Natl Ctr Engn Res Vet Bioprod, Key Lab Vet Biol Engn & Technol, Inst Vet Med,Minist

关键词： Adhesion;Mycoplasma hyorhinis;Variable lipoprotein

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Mycoplasma hyorhinis (M. hyorhinis) is a swine pathogen that is associated with various human cancers and contamination in cell cultures. However, no studies on the adhesion molecules of this pathogen have yet been reported. The variable lipoprotein (Vlp) family is an important surface component of M. hyorhinis. Herein, we performed several experiments to identify the function of the Vlp family in adherence to host cells. Seven recombinant Vlp (rVlp) proteins were expressed in Escherichia coli and purified by affinity chromatography. The potential role of rVlp adherence to pig kidney (PK-15) and swine tracheal epithelial (STEC) cells was then studied by indirect immunofluorescence assay and microtiter plate adherence assay. Adhesion of M. hyorhinis to PK-15 and STEC cells was specifically inhibited by the addition of a cocktail of rVIp proteins. The rVlp protein mixture was shown to bind to both PK-15 and STEC cells. The binding increased in a dose-dependent manner and could be blocked by antisera against the rVlp proteins. Most of the rVlp proteins could bind individually to both PK-15 and STEC cells except for rVlpD and rVlpF, which bound only to STEC cells. Because Vlp members vary in size among different strains and generations, they may vary in their cytoadhesion capabilities in various strains. In summary, the present results indicate that the Vlp family functions as adhesins of M. hyorhinis. (C) 2016 Published by Elsevier B.V.

  • 相关文献

[1]Characterization of the role in adherence of Mycoplasma hyorhinis variable lipoproteins containing different repeat unit copy numbers. Xiong, Qiyan,Zhang, Bixiong,Wang, Jia,Ni, Bo,Ji, Yan,Wei, Yanna,Feng, Zhixin,Liu, Maojun,Shao, Guoqing,Zhang, Bixiong,Xiao, Shaobo,Shao, Guoqing.

[2]E-coli recA gene improves gene targeted homologous recombination in Mycoplasma hyorhinis. Ishag, Hassan Z. A.,Xiong, Qiyan,Liu, Maojun,Feng, Zhixin,Shao, Guoqing,Ishag, Hassan Z. A..

[3]GFP as a marker for transient gene transfer and expression in Mycoplasma hyorhinis. Ishag, Hassan Z. A.,Liu, Maojun,Yang, Ruosong,Xiong, Qiyan,Feng, Zhixin,Shao, Guoqing,Ishag, Hassan Z. A.. 2016

[4]Development of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Mycoplasma hyorhinis. Du, Gai-Mei,Liu, Mao-Jun,Wu, Yu-Zi,Xiong, Qi-Yan,Bai, Fang-Fang,Feng, Zhi-Xin,Shao, Guo-Qing,Du, Gai-Mei. 2013

[5]Adhesion capability of first two domains at N terminus of NP_785232 protein and their interaction with a UV-absorbing component from human mucus. Du, L.,Huo, G.,Du, L.,Ju, X.,Liu, F.. 2010

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台