您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Evaluation of immune effect to recombinant potential protective antigens of Mycoplasma ovipneumoniae in mice

文献类型： 外文期刊

作者： Chen, Yi 1 ; Wang, Xiaonan 1 ; Chen, Siyu 1 ; Zhang, Mengjie 1 ; Cheng, Zilong 1 ; Zhang, Wenwen 1 ; Liu, Diyue 1 ; Shan, Yiyi 1 ; Du, Gaimei 4 ; Li, Wenliang 1 ; Yang, Leilei 1 ; Wang, Jinquan 3 ; Chu, Yuefeng 2 ; Liu, Maojun 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Vet Med, Key Lab Vet Bioprod Engn, Minist Agr & Rural Affairs, Nanjing 210014, Peoples R China

2.Lanzhou Univ, Lanzhou Vet Res Inst, Chinese Acad Agr Sci, Coll Vet Med,State Key Lab Anim Dis Control & Prev, Lanzhou 730000, Peoples R China

3.Xinjiang Agr Univ, Coll Vet Med, Urumqi 830052, Peoples R China

4.Jinling Inst Technol, Coll Anim Sci & Food Engn, Nanjing 211169, Peoples R China

5.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Peoples R China

6.Guotai Technol Innovat Ctr Vet Bioprod Taizhou, Taizhou 225300, Peoples R China

关键词： Mycoplasma ovipneumoniae; Eno; Immune response; Mice; Protective effect

期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.5； 五年影响因子：3.6 ）

ISSN： 0882-4010

年卷期： 2025 年 204 卷

页码：

收录情况： SCI

摘要： Mycoplasma ovipneumoniae is a primary causative agent of pneumonia in ruminants, causing chronic nonprogressive pneumonia in domestic sheep and goats, but leading to higher morbidity and mortality in bighorn sheep and wild small ruminants. This disease has become a widespread epidemic, resulting in significant losses to the sheep industry. In this study, we evaluated the immunogenicity and initial protective effects of four antigenic proteins of M. ovipneumoniae, namely Eno, EF-Tu, Ulad, and T4SS. These proteins were used to immunize BALB/c mice either individually or in a combination (rProteins group). The mice were intranasally infected with 109 CCU50/mL M. ovipneumoniae strain NJ01 twice, on days 28 and 30 after immunization. Among the four recombinant proteins, rEno demonstrated the most promising results in terms of inducing specific humoral and cellular immune responses. It also resulted in the lowest lung lesion scores and the lowest M. ovipneumoniae loads in the lungs and bronchoalveolar lavage fluid (BALF). Compared to the other three proteins, rEno provided superior protection. Furthermore, the rEno vaccine significantly reduced the inflammatory response in the lungs of mice, as evidenced by the evaluation of pro-inflammatory cytokines. The expression of IL-1 beta and NF-kappa B was significantly reduced, while the expression of IL-4 was significantly increased. In conclusion, the rEno vaccine elicited a favorable immunological response and conferred protection against M. ovipneumoniae. This finding presents a novel approach to controlling the global spread of this pathogen.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台