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The anaphase-promoting complex initiates zygote division in Arabidopsis through degradation of cyclin B1

文献类型： 外文期刊

作者： Guo, Lei 1 ; Jiang, Li 1 ; Zhang, Ying 1 ; Lu, Xiu-Li 1 ; Xie, Qi 3 ; Weijers, Dolf 4 ; Liu, Chun-Ming 1 ;

作者机构： 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China

2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

3.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China

4.Wageningen Univ, Biochem Lab, Dreijenlaan 3, NL-6703 HA Wageningen, Netherlands

5.Chinese Acad Fishery Sci, Beijing 100141, Peoples R China

关键词： APC11;cyclosome;zygote activation;cell cycle;ubiquitination;Arabidopsis thaliana

期刊名称：PLANT JOURNAL （ 影响因子：6.417； 五年影响因子：7.627 ）

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收录情况： SCI

摘要： As the start of a new life cycle, activation of the first division of the zygote is a critical event in both plants and animals. Because the zygote in plants is difficult to access, our understanding of how this process is achieved remains poor. Here we report genetic and cell biological analyses of the zygote-arrest 1 (zyg1) mutant in Arabidopsis, which showed zygote-lethal and over-accumulation of cyclin B1 D-box-GUS in ovules. Map-based cloning showed that ZYG1 encodes the anaphase-promoting complex/cyclosome (APC/C) subunit 11 (APC11). Live-cell imaging studies showed that APC11 is expressed in both egg and sperm cells, in zygotes and during early embryogenesis. Using a GFP-APC11 fusion construct that fully complements zyg1, we showed that GFP-APC11 expression persisted throughout the mitotic cell cycle, and localized to cell plates during cytokinesis. Expression of non-degradable cyclin B1 in the zygote, or mutations of either APC1 or APC4, also led to a zyg1-like phenotype. Biochemical studies showed that APC11 has self-ubiquitination activity and is able to ubiquitinate cyclin B1 and promote degradation of cyclin B1. These results together suggest that APC/C-mediated degradation of cyclin B1 in Arabidopsis is critical for initiating the first division of the zygote.

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