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Comparative proteomic analysis of latex from Hevea brasiliensis treated with Ethrel and methyl jasmonate using iTRAQ-coupled two-dimensional LC-MS/MS

文献类型： 外文期刊

作者： Dai, Longjun 1 ; Kang, Guijuan 1 ; Nie, Zhiyi 1 ; Li, Yu 1 ; Zeng, Rizhong 1 ;

作者机构： 1.Minist Agr, Key Lab Biol & Genet Resources Rubber Tree, Shanghai, Peoples R China; CATAS, Rubber Res Inst, State Key Lab Incubat Base, Danzhou, Hainan, Peoples R China

关键词： Ethrel;Hevea brasiliensis;iTRAQ;Latex;Methyl jasmonate;Proteomics

期刊名称：JOURNAL OF PROTEOMICS （ 影响因子：4.044； 五年影响因子：4.02 ）

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收录情况： SCI

摘要： Ethrel (ET) is an effective and widely used latex yield stimulant of Hevea brasiliensis (Para rubber tree), and jasmonate (JA) is a key inducer of laticifer differentiation in this plant. To examine variations in the latex proteome caused by these phytohormones, ET and methyl jasmonate (MeJA) were applied to Reyan 7-33-97 rubber tree clones, and comparative proteomic analyses were conducted. On the basis of a transcriptome shotgun assembly (TSA) sequence database and an iTRAQ-coupled two-dimensional LC MS/MS approach, 1499 latex proteins belonging to 1078 clusters were identified. With a 1.5-fold cut-off value to determine up- and down-regulated proteins, a total of 101 latex proteins were determined to be regulated by ET and/or MeJA via pairwise comparisons among the three exposure durations (0 h, 6 h, and 48 h). Proteins associated with latex regeneration, including phosphoenolpyruvate carboxylase and acetyl-CoA C-acetyltransferase, and those associated with latex flow, such as chitinase and a sieve element occlusion protein, were affected by the application of ET. Chitinase and polyphenol oxidase were also found to be regulated by MeJA. The findings of this study may provide new insight into the roles of phytohormones in latex yield and the causative mechanisms of laticifer differentiation in rubber trees.

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