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Up-regulated expression of Ran reveals its potential role to deltamethrin stress in Kc cells

文献类型： 外文期刊

作者： Liu, Wei 1 ; Xu, Qin 2 ; Chi, Qingping 1 ; Hu, Junli 1 ; Li, Fengliang 3 ; Cheng, Luogen 1 ;

作者机构： 1.Nanjing Normal Univ, Coll Life Sci, Jiangsu Key Lab Biodivers & Biotechnol, Nanjing 210046, Jiangsu, Peoples R China

2.Nanjing Normal Univ, Sch Energy & Mech Engn, Nanjing 210042, Jiangsu, Peoples R China

3.Guizhou Acad Agr, Inst Plant Protect, Guiyang 550009, Peoples R China

关键词： Ran;Deltamethrin;Stress reaction;Kc cells

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

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收录情况： SCI

摘要： The GTP-binding nuclear protein Ran has mostly been reported to be an essential player in nuclear transport, chromosome alignment, microtubule dynamics, centrosome duplication, kinetochore attachment of microtubules, nuclear-envelope dynamics, and phagocytosis. However, until now, there has been no report showing the involvement of Ran in DM stress. In this paper, two-dimensional electrophoresis analysis showed that the expression level of Ran in Kc cells in response to DM was higher than that in the control group. In addition, quantitative analysis using real-time PCR revealed that the expression of Ran was obviously up-regulated at various concentrations of DM. Western blot analysis showed that Ran was up-regulated 2.27-fold over the control at 48 h. Because we still could not pinpoint whether Ran was actually involved in DM stress reaction, to further verify the role of Ran in stress reaction, RNA interference and cell transfection were utilized. Overexpression of Ran in cells conferred a degree of protection against DM after 72 h. Furthermore, interference with Ran significantly decrease cell viability. All of the above findings strongly imply that Ran may participate in the development of stress reaction to DM. Therefore, investigating the possible role of Ran in DM stress will broaden our limited knowledge regarding DM stress inducible genes. (C) 2016 Elsevier B.V. All rights reserved.

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[1]The identification and characterisation of a new deltamethrin resistance-associated gene, UBL40, in the diamondback moth, Plutella xylostella (L.). Zhang, Hong,Cheng, Chen,Jiao, Dongxu,Zhou, Zhou,Cheng, Luogen,Li, Fengliang.

[2]PROTEOMIC ANALYSIS OF UBIQUITINATED PROTEINS FROM DELTAMETHRIN-RESISTANT AND SUSCEPTIBLE STRAINS OF THE DIAMONDBACK MOTH, Plutella Xylostella L.. Cheng, Luogen,Du, Yaqiong,Hu, Junli,Jiao, Dongxu,Li, Jin,Zhou, Zhou,Xu, Qin,Li, Fengliang.

[3]IDENTIFICATION OF PROTEASOME ALPHA6 SUBUNIT ASSOCIATED WITH DELTAMETHRIN RESISTANCE IN Drosophila melanogaster Kc CELLS. Hu, Junli,Chi, Qingping,Liu, Wei,Cheng, Luogen,Xu, Qin,Li, Fengliang.

[4]Analysis of UB and L40 resistance related to deltamethrin in Drosophila kc cells. Chi, Qingping,Liu, Wei,Hu, Junli,Cheng, Luogen,Li, Fengliang,Cheng, Ying,Jing, Xuejian,Xu, Qin.

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