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In Vitro Evaluation of Swine-Derived Lactobacillus reuteri: Probiotic Properties and Effects on Intestinal Porcine Epithelial Cells Challenged with Enterotoxigenic Escherichia coli K88

文献类型： 外文期刊

作者： Wang, Zhilin 1 ; Wang, Li 1 ; Chen, Zhuang 1 ; Ma, Xianyong 1 ; Yang, Xuefen 2 ; Zhang, Jian; Jiang, Zongyong;

作者机构： 1.Guangdong Acad Agr Sci, Key Lab Anim Nutr & Feed Sci South China,State Ke, Guangdong Publ Lab Anim Breeding & Nutr,Minist Ag, Guangdong Key Lab Anim Breeding & Nutr,Inst Anim, Guangzhou 510640, Guangdong, Peoples R China

2.Guangdong Acad Agr Sci, Key Lab Anim Nutr & Feed Sci South China,State Ke, Guangdong Publ Lab Anim Breeding & Nutr,Minist Ag, Guangdong

关键词： Lactobacillus reuteri;in vitro evaluation;probiotic properties;enterotoxigenic Escherichia coli

期刊名称：JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：2.351； 五年影响因子：2.65 ）

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收录情况： SCI

摘要： Probiotics are considered as the best effective alternatives to antibiotics. The aim of this study was to characterize the probiotic potential of lactobacilli for use in swine farming by using in vitro evaluation methods. A total of 106 lactic acid bacterial isolates, originating from porcine feces, were first screened for the capacity to survive stresses considered important for putative probiotic strains. Sixteen isolates showed notable acid and bile resistance, antibacterial activity, and adherence to intestinal porcine epithelial cells (IPEC-1). One isolate, LR1, identified as Lactobacillus reuteri, was selected for extensive study of its probiotic and functional properties in IPEC-1 cell models. L. reuteri LR1 exhibited good adhesion to IPEC-1 cells and could inhibit the adhesion of enterotoxigenic Escherichia coli (ETEC) to IPEC-1 cells. L. reuteri LR1 could also modulate transcript and protein expression of cytokines involved in inflammation in IPEC-1 cells; the Lactobacillus strain inhibited the ETEC-induced expression of proinflammatory transcripts (IL-6 and TNF-alpha) and protein (IL-6), and increased the level of anti-inflammatory cytokine (IL-10). Measurement of the permeation of FD-4 showed that L. reuteri LR1 could maintain barrier integrity in monolayer IPEC-1 cells exposed to ETEC. Immunolocalization experiments showed L. reuteri LR1 could also prevent ETEC-induced tight junction ZO-1 disruption. Together, these results indicate that L. reuteri LR1 exhibits desirable probiotic properties and could be a potential probiotic for use in swine production.

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