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Two novel soluble trehalase genes cloned from Harmonia axyridis and regulation of the enzyme in a rapid changing temperature

文献类型： 外文期刊

作者： Shi, Zuokun 1 ; Liu, Xiaojun 1 ; Xu, Qingye 1 ; Qin, Zi 1 ; Wang, Su 2 ; Zhang, Fan 3 ; Wang, Shigui 1 ; Tang, Bin 1 ;

作者机构： 1.Hangzhou Normal Univ, Coll Life & Environm Sci, Hangzhou Key Lab Anim Adaptat & Evolut, Hangzhou 310036, Zhejiang, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100089, Peoples R China

3.Beijing Acad Agr & Forestry Sci, Inst

关键词： Cold resistance;Harmonia axyridis;Molecular cloning;Soluble trehalase;Trehalose

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY （ 影响因子：2.231； 五年影响因子：2.215 ）

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收录情况： SCI

摘要： In previous studies, we have cloned two soluble trehalase genes (HaTreh1-1 and HaTreh1-2) from the harlequin ladybird Harmonia axyridis. Here, we obtained the other two novel genes (HaTreh1-3 and HaTreh1-4) by transcriptome sequencing and rapid amplification of cDNA ends. Generally, anabolism enhancement and catabolism inhibition together contribute to accumulation of trehalose, and trehalase is the key enzyme to start the catabolism of trehalose. To characterize the metabolism of trehalose in H. axyridis and how these trehalase genes are regulated under cold stress conditions, a comparison of trehalose content and trehalase levels in two different rapidly changing temperature environments was carried out to explore the regulation of these genes. We found that an accumulation of trehalose could be observed at 5 degrees C, 0 degrees C and -5 degrees C and trehalase was suppressed in these temperature points during a gradually cooling environment. Then, in a gradually warming environment, trehalose levels increased slightly from -5 degrees C to -15 degrees C and then decreased at 25 degrees C; however, no significant negative association was observed between trehalase and trehalose. Additionally, we found that glycogen could be converted into trehalose to help the individual resist the low temperature. Analysis of the expression of soluble trehalase showed that HaTreh1-1, HaTreh1-2, HaTreh1-3 and HaTreh1-4 were involved in trehalose metabolism; but the gene HaTreh1-4 plays the most important role in the cooling process, and HaTreh1-2 and HaTreh1-4 play the most important role in the warming process. Finally, we found that 5 degrees C might be a temperature signal for H. axyridis; prior to this temperature, individuals must make enough physical preparations to resist cold stress during the winter. (C) 2016 Elsevier Inc All rights reserved.

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[1]Trehalase in Harmonia axyridis (Coleoptera: Coccinellidae): effects on beetle locomotory activity and the correlation with trehalose metabolism under starvation conditions. Tang, Bin,Qin, Zi,Wang, Su,Guo, Xiao-Jun,Zhang, Fan,Tang, Bin,Shi, Zuo-Kun,Wang, Shi-Gui.

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