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The developmental effect of difenoconazole on zebrafish embryos: A mechanism research

文献类型: 外文期刊

作者: Mu, Xiyan 1 ; Chai, Tingting 1 ; Wang, Kai 3 ; Zhu, Lizhen 1 ; Huang, Ying 2 ; Shen, Gongming 2 ; Li, Yingren 2 ; Li, Xue 1 ;

作者机构: 1.China Agr Univ, Coll Sci, Beijing 100094, Peoples R China

2.Chinese Acad Fishery Sci, Ctr Fishery Resources & Ecol Environm Res, Beijing, Peoples R China

3.Shenyang Agr Univ, Plant Protect Coll, Shenyang 110161, Peoples R China

关键词: Zebrafish;Difenoconazole;Developmental effect;Embryo;Lipid

期刊名称:ENVIRONMENTAL POLLUTION ( 影响因子:8.071; 五年影响因子:8.35 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Difenoconazole is a widely used triazole fungicide and has been reported to have negative impacts on zebrafish embryos. To investigate the mechanism of its developmental toxicity, zebrafish embryos were exposed to 0.5 and 2.0 mg/L difenoconazole for 96 h. The morphological and physiological indicators of embryo development were tested. The total cholesterol (TCHO) level, triglyceride (TG) level and malondialdehyde (MDA) content were measured at 96 hpf (hours post-fertilization). In addition, the transcription of genes related to embryo development, the antioxidant system, lipid synthesis and metabolism was quantified. Our results showed that a large suite of symptoms were induced by difenoconazole, including hatching regression, heart rate decrease, growth inhibition and teratogenic effects. 0.5 mg/L difenoconazole could significantly increase the TG content of zebrafish embryos at 96 hpf, while no apparent change in the TCHO and MDA level was observed post 96 h exposure. Q-PCR (quantitative real-time polymerase chain reaction) results showed that the transcription of genes related to embryonic development was decreased after exposure. Genes related to hatching, retinoic acid metabolism and lipid homeostasis were up-regulated by difenoconazole. (C) 2016 Elsevier Ltd. All rights reserved.

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