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Identification and role analysis of an intermediate produced by a polygenic mutant of Monascus pigments cluster in Monascus ruber M7

文献类型： 外文期刊

作者： Liu, Jiao 1 ; Zhou, Youxiang 2 ; Yi, Tao 1 ; Zhao, Mingming 2 ; Xie, Nana 4 ; Lei, Ming 1 ; Liu, Qingpei 1 ; Shao, Yanchu 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China

2.Hubei Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Wuhan 430070, Peoples R China

3.Huazhong Agr Univ, Key Lab Environm Correlat Dietol, Minist Educ, Wuhan 430070, Peoples R China

4.Chongqing Technol & Business Univ, Sch Environm & Biol Engn, Chongqing 400067, Peoples R China

关键词： Monascus pigment;Pigment intermediate;Biosynthesis pathway;Monascus ruber

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

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收录情况： SCI

摘要： Monascus pigments (Mps) are a group of azaphilonic secondary metabolites produced by Monascus spp. via a polyketide pathway. A mutant deleted an about 30 kb region of Mps gene cluster from Monascus ruber M7 was isolated previously, which produces a high amount of a light yellow pigment. The current study revealed that the mutant named Delta MpigJ-R lost proximate eight genes of the Mps gene cluster in M. ruber M7 through genetic analysis at DNA and RNA levels. The produced light yellow material was identified as a benzaldehyde derivative named as 6-(4-hydroxy-2-oxopentyl)-3-methyl-2, 4-dioxocyclohexane carb-aldehyde (M7PKS-1) by FT-IR, NMR, and MS. The sodium acetate-1-C-13 feeding experiment indicated that M7PKS-1 was a product produced from polyketide pathway. Finally, the feeding of M7PKS-1 helped to induce and regain Mps production of the mutants (Delta MpigA and Delta MpigE) which were previously unable to biosynthesize Mps and proved that M7PKS-1 was an initial intermediate of Mps. The results in this study provide a line of action to unveil Monascus pigments biosynthesis pathway.

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[1]mrskn7, a putative response regulator gene of Monascus ruber M7, is involved in oxidative stress response, development, and mycotoxin production. Shao, Yanchun,Chen, Fusheng,Yang, Sha,Zhang, Zhouwei,Zhou, Youxiang.

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[3]Effects of cyclic AMP on development and secondary metabolites of Monascus ruber M-7. Chen, F.,Chen, F.,Lai, Y.,Wang, L.,Qing, L.,Chen, F.. 2011

[4]NAD(+)-dependent HDAC inhibitor stimulates Monascus pigment production but inhibit citrinin. Hu, Yan,Mao, Zejing,Chen, Fusheng,Shao, Yanchun,Zhou, Youxiang,Li, Huihui. 2017

[5]Effects of an alternative oxidase gene on conidia viability under external stresses in Monascus ruber M7. Shao, Yanchun,Li, Qi,Chen, Fusheng,Zhou, Youxiang,Chen, Fusheng. 2017

[6]Identification of Mga1, a G-protein alpha-subunit gene involved in regulating citrinin and pigment production in Monascus ruber M7. Li, Li,Shao, Yanchun,Li, Qi,Yang, Sha,Chen, Fusheng,Shao, Yanchun,Chen, Fusheng,Chen, Fusheng. 2010

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