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Pathogenic characteristics of a novel triple-reasserted H1N2 swine influenza virus

文献类型： 外文期刊

作者： Liu, Huili 1 ; Tao, Jie 1 ; Zhang, Pengchao 1 ; Yin, Xiuchen 1 ; Ha, Zhuo 1 ; Zhang, Chunling 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Anim Sci & Vet Med, Shanghai 201106, Peoples R China

2.Municipal Key Lab Agrigenet & Breedings, Shanghai 201106, Peoples R China

关键词： Swine influenza virus;H1N2;Pathogenicity

期刊名称：BIOLOGICALS （ 影响因子：1.856； 五年影响因子：2.05 ）

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收录情况： SCI

摘要： A novel triple reasserted H1N2 virus A/swine/Shanghai/1/2007 (SH07) was isolated from nasal swabs of weaned pig showing clinical symptoms of coughing and sneezing. To explore the virus characteristics, mice, chickens and pigs were selected for pathogenicity study. Pigs inoculated intranasally with 10(6) TCID50 SHO7 showed clinical symptoms with coughing and sneezing, but no death. The virus nuclear acid was detected in many tissues using real-time PCR, which was mainly distributed in respiratory system particularly in the lungs. The virus was low-pathogenic to chickens with 10(6) TCID50 dose inoculation either via intramuscular or intranasal routes. However virus nuclear acid detection and virus isolation confirmed that the virus can also be found in nasal and rectum. When virus was inoculated into mice by intramuscular or intranasal routes we observed 100% and 80% lethality respectively. The third generation of samples passaged on MDCK cell were SIV positive in indirect immunofluorescence assay (IFA) using antiserum against H1N2 SIV. Furthermore, the lungs of mice showed obvious lesion with interstitial pneumonia. Data in our study suggest that SHO7 is preferentially pathogenic to mammals rather than birds although it is a reasserting virus with the fragments from swine, human and avian origin. (C) 2016 International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.

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