文献类型： 外文期刊

作者： Shui, Y. ¹ ; Xu, Z. H. ¹ ; Shi, Y. H. ² ; Zhou, X. ¹ ;

作者机构： 1.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Minist Agr, Key Lab Freshwater Fisheries & Germplasm Resource, Wuxi 214081, Peoples R China

2.Chinese Acad Agr Sci, Shanghai Vet Res Inst, Shanghai 200241, Peoples R China

3.Nanjing Agr Univ, Wuxi Fishery Coll, Wuxi 214081, Peoples R China

期刊名称：CRUSTACEANA （ 影响因子：0.529； 五年影响因子：0.65 ）

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收录情况： SCI

摘要： The maturation promoting factor (MPF), a complex of CDC2 (CDK1) and cyclin B, is a key regulator of controlling the G2/M phase transition in the meiotic maturation of the oocyte in multi cellular organisms. In this study, full-length cDNA of cyclin B (Pc-cyclin B) from the red swamp crayfish, Procambarus clarkii, was cloned using the degenerate RT-PCR and RACE methods. The cDNA of Pc-cyclin B is 2595 bp in length and encoding a protein of 402 amino acids, with a calculated molecular mass of 45.75 kDa. Six potential cytoplasmic polyadenylation elements (CPE) as well as one signal sequences (AATAAA) were found in the 3'-UTR location. Semi-quantitative RT-PCR analysis revealed that the amount of cyclin B mRNA was highest in the ovary, followed by the heart (P <0.05); and also significantly higher in the pre-vitellogenesis (pre-Vt) and primary-vitellogenesis (pVt) stages, while low in the tertiary-vitellogenesis and GVBD stages (P <0.05), suggesting that differential expression of Pc-cyclin B is closely related to oogonial proliferation (mitosis) and oocyte meiotic maturation in this species of crayfish.