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Two AMT2-Type Ammonium Transporters from Pyrus betulaefolia Demonstrate Distinct Expression Characteristics

文献类型： 外文期刊

作者： Li, Hui 1 ; Cong, Yu 2 ; Chang, You-hong 1 ; Lin, Jing 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Hort, Jiangsu Key Lab Hort Crop Genet Improvement, 50 Zhongling St, Nanjing 210014, Jiangsu, Peoples R China

2.Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, 71 East Beijing Rd, Nanjing 210008, Jiangsu, Peoples R China

关键词： Pyrus betulaefolia;AMT2-type ammonium transporter;Gene characteristic;Regulatory factor

期刊名称：PLANT MOLECULAR BIOLOGY REPORTER （ 影响因子：1.595； 五年影响因子：2.042 ）

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收录情况： SCI

摘要： Ammonium transporters (AMTs) play dominant roles in ammonium transport and distribution across cellular membranes. However, the AMT2-type AMTs from pear remain poorly understood. To characterize ammonium transport pathways in Pyrus betulaefolia Bunge, one popular rootstock for pear and two new genes with high homology to AMT2-type AMTs, PbAMT2 and PbAMT3, were isolated from its seedlings. The analysis of the PbAMT expression patterns in different organs of P. betulaefolia showed that expression of a given transporter could be very specific and their regulatory mechanisms were distinct. Both responded to various nitrogen regimes, PbAMT2 was detected in all organs, and the highest transcription was found in roots, whereas PbAMT3 was restricted to leaves. In addition, transcript level of PbAMT2 was strongly affected by the diurnal cycle, and PbAMT3 was exclusively expressed in leaves after treatment with two phytohormones: abscisic acid and methyl jasmonate. A complementation experiment using the yeast strain 31019b revealed that both PbAMT2 and PbAMT3 encoded functional, high-affinity AMTs. Furthermore, uptake studies indicated that these two AMTs possessed disparate affinities for ammonium and had opposite responses to external pH change. In summary, the present study provides basic genomic, transcript information and nitrogen transporter features for the pear AMT2 subfamily; indeed, PbAMT2 and PbAMT3 demonstrated distinct expression characteristics, which will pave the way for deciphering the high-affinity ammonium transport system in pear.

  • 相关文献

[1]Gene characterization and transcription analysis of two new ammonium transporters in pear rootstock (Pyrus betulaefolia). Li, Hui,Han, Jin-Long,Chang, You-Hong,Lin, Jing,Yang, Qing-Song.

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