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Effects of heat stress on serum cortisol, alkaline phosphatase activity and heat shock protein 40 and 90 beta mRNA expression in rainbow trout Oncorhynchus mykiss

文献类型： 外文期刊

作者： Li, Zhen 1 ; Liu, Zhe 1 ; Wang, Yan Ni 1 ; Kang, Yu Jun 1 ; Wang, Jian Fu 1 ; Shi, Hai Na 1 ; Huang, Jin Qiang 1 ; Jiang, 1 ;

作者机构： 1.Gansu Agr Univ, Coll Anim Sci & Technol, 1 Yingmencun, Lanzhou 730070, Gansu, Peoples R China

2.Chinese Acad Fishery Sci, 150 Nanqingtacun, Beijing 100039, Peoples R China

关键词： Oncorhynchus mykiss;heat stress;heat shock protein 40;heat shock protein 90 beta;cortisol;alkaline phosphatase activity

期刊名称：BIOLOGIA （ 影响因子：1.35； 五年影响因子：1.139 ）

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收录情况： SCI

摘要： The enhanced expression of heat shock proteins (Hsps) in organisms is associated with environmental temperatures. For rainbow trout (Oncorhynchus mykiss), high temperature is a major abiotic stress limiting its growth and productivity. In the present study, we have investigated serum cortisol (COR) and alkaline phosphatase (AKP) activity as well as Hsp40 and Hsp90 beta mRNA expression in different tissues (gill, liver, spleen, heart, brain and head kidney) of rainbow trout under unstressed (18 degrees C) and heat-stressed (25 degrees C) conditions. Consequently, COR at 8 H, and heat stress recovering (HSR) AKP at 2, 8 and 12 H were significantly up-regulated in the heat stressed group (P < 0.05). Hsp40 and Hsp90 beta mRNA expressions were significantly up-regulated in all tissues and responded to heat stress in a tissue-and time-specific manner. Their expression in heart and brain were relatively high in the unstressed group compared with other tissues (P < 0.05). During heat stress, Hsp40 and Hsp90 beta in all tissues showed the first rise after falling trend, and the highest induction was recorded in the spleen (P < 0.05). Hsp40 in heart and brain, and Hsp90 beta in liver and brain did not return to the comparison group (0 h) after HSR. The results indicate that Hsp40 and Hsp90 beta are more important in dealing with heat-induced stress and mainly afforded protection in liver, heart and brain. In addition, cells may be damaged under heat stress with increasing AKP activity and elevating COR, which regulates energy metabolism and fights environmental pressure to maintain homeostasis of rainbow trout.

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