Cyprinid herpesvirus 2 infection changes microbiota and metabolites in the gibel carp (Carassius auratus gibelio) midgut
文献类型： 外文期刊
作者： Chen, Peng 1 ; Zhang, Mingming 1 ; Zhang, Yichan 1 ; Li, Jun 3 ; Wan, Xihe 4 ; Lv, Tingli 1 ; Chen, Yiyue 1 ; Zhao, Zhigang 2 ; Ma, Zhihao 1 ; Zhu, Zhu 5 ; Chen, Lihua 6 ; Li, Zhen 1 ; Wang, Zisheng 1 ; Qiao, Guo 1 ;
作者机构： 1.Yancheng Inst Technol, Res Ctr Aquat Anim Immun & Dis Control, Yancheng, Jiangsu, Peoples R China
2.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Heilongjiang Prov Key Lab Cold Water Fish Germplas, Harbin, Peoples R China
3.Florida Int Univ, Biomol Sci Inst, Dept Biol Sci, Miami, FL USA
4.Inst Marine Fisheries, Cent Key Lab Jiangsu, Nantong, Jiangsu, Peoples R China
5.Ctr Fisheries Technol Popularizat Sheyang Agr, Rural Bur, Yancheng, Jiangsu, Peoples R China
6.Hohai Univ, Coll Agr Sci & Engn, Nanjing, Peoples R China
关键词： CyHV-2; gut microbiota-mediated metabolites; Aeromonas; Cetobacterium; tryptophan metabolism
期刊名称：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY （ 影响因子：5.7； 五年影响因子：5.9 ）
ISSN： 2235-2988
年卷期： 2023 年 12 卷
页码：
收录情况： SCI
摘要： Cyprinid herpesvirus 2 (CyHV-2) infects gibel carp (Carassius auratus gibelio) and causes severe losses. Microbiota in animal guts involves nutrition intake, development, immunity, and disease resistance. However, the relationship between gibel carp gut microbiota and CyHV-2 infection is not well known. Herein, we analyzed the gut microbiota composition and metabolite profiles in CyHV-2-infected and -uninfected fish using high-throughput sequencing and gas chromatography/mass spectrometry. Results showed that CyHV-2 infection significantly changed gut microbiota and metabolite profiles (p < 0.05). High-throughput sequencing demonstrated that the relative abundance of Aeromonas in the midgut increased dramatically while Cetobacterium decreased. Time-course analysis showed that the number of Aeromonas in the midgut of infected fish increased more than 1,000 times within 5 days post infection. Metabolome analysis illustrated that CyHV-2 infection significantly altered 24 metabolites in the midgut of gibel carp, annotating to the anomaly of digestion and metabolisms of amino acids, carbohydrates, and lipids, such as tryptophan (Trp) metabolism. The Mantel test demonstrated that gut microbiota and metabolite profiles were well related (r = 0.89). Furthermore, Trp metabolism responded to CyHV-2 infection closely was taken as one example to prove the correlation among CyHV-2 infection, metabolites and microbiota in the midgut, and host immunity. Results showed that modulating Trp metabolism could affect the relative abundance of Aeromonas in the midgut of fish, transcription of antiviral cytokines, and CyHV-2 infection. Therefore, we can conclude that CyHV-2 infection significantly perturbed the gut microbiome, disrupted its' metabolic functions, and caused the proliferation of the opportunistic pathogen Aeromonas. This study also suggests that modulation of the gut microbiome will open a therapeutic opportunity to control CyHV-2 infection in gibel carp.
- 相关文献
作者其他论文 更多>>
-
Evaluation of the ecological carrying capacity of Wailingding marine ranching in Zhuhai, China by high-resolution remote sensing
作者：Wang, Ziwen;Li, Zhen;Wang, Ziwen;Yu, Jing;Chen, Pimao;Li, Zhen;Yang, Weiwei;Wang, Ziwen;Yu, Jing;Chen, Pimao;Li, Zhen;Yang, Weiwei;Yao, Lijun;Yang, Weiwei
关键词：marine ecological carrying capacity; marine ranching; high-resolution remote sensing; Wailingding; three-dimensional state-space model; weighted Bonferroni mean
-
The Biochemical Composition and Quality of Adult Chinese Mitten Crab Eriocheir sinensis Reared in Carbonate-Alkalinity Water
作者：Wang, Shihui;Luo, Liang;Zhang, Rui;Guo, Kun;Zhao, Zhigang;Wang, Shihui;Luo, Liang;Zhang, Rui;Guo, Kun;Zhao, Zhigang
关键词：Eriocheir sinensis; saline-alkaline; aquaculture; edible yield; mineral element
-
Hypolipidemic Effect of Rice Bran Oil Extract Tocotrienol in High-Fat Diet-Induced Hyperlipidemia Zebrafish (Danio Rerio) Induced by High-Fat Diet
作者：Liu, Naicheng;Zhang, Peng;Xue, Mingyang;Zhang, Mengwei;Huang, Zhenyu;Xu, Chen;Meng, Yan;Fan, Yuding;Liu, Wei;Zhang, Feixiang;Zhou, Yong;Zhang, Feixiang;Chen, Peng
关键词：rice bran oil extract; tocotrienol (T3); zebrafish; type 2 diabetes mellitus (T2DM) model; high-fat diet; blood fat
-
The Effect of Artificial Substrate and Carbon Source Addition on Bacterial Diversity and Community Composition in Water in a Pond Polyculture System
作者：Guo, Kun;Shi, Mumin;Ruan, Guoliang;Zhao, Zhigang;Guo, Kun;Shi, Mumin;Huang, Xiaoli;Luo, Liang;Wang, Shihui;Zhang, Rui;Xu, Wei;Zhao, Zhigang;Guo, Kun;Shi, Mumin;Huang, Xiaoli;Luo, Liang;Wang, Shihui;Zhang, Rui;Xu, Wei;Zhao, Zhigang
关键词：artificial substrate; biofloc; 16S rRNA; high-throughput sequencing; microbial community
-
Ginger polysaccharide alleviates the effects of acute exposure to carbonate in crucian carp (Carassius auratus) by regulating immunity, intestinal microbiota, and intestinal metabolism
作者：Meng, Xianwei;Luo, Liang;Zhao, Zhigang;Wang, Shihui;Zhang, Rui;Guo, Kun;Meng, Xianwei;Luo, Liang
关键词：Ginger polysaccharide; Carassius auratus; Carbonate alkalinity stress; Intestinal microbiota; Intestinal metabolism
-
Untargeted LC-MS metabolomics reveals the metabolic responses in the Eriocheir sinensis gills exposed to salinity and alkalinity stress
作者：Wang, Shihui;Song, Yingying;Luo, Liang;Zhang, Rui;Guo, Kun;Zhao, Zhigang;Wang, Shihui;Luo, Liang;Zhang, Rui;Guo, Kun;Zhao, Zhigang;Song, Yingying;Zhao, Zhigang
关键词：Eriocheir sinensis; Saline-alkaline; Fatten; Metabolomics
-
Correlation analysis of phosphorylation of myofibrillar protein and muscle quality of tilapia during storage in ice
作者：Yu, Ye;Shi, Wenzheng;Wei, Ya;Chen, Shengjun;Wang, Yueqi;Huang, Hui;Li, Chunsheng;Wang, Di;Zhao, Yongqiang;Wei, Ya;Chen, Shengjun;Wang, Yueqi;Huang, Hui;Li, Chunsheng;Wang, Di;Zhao, Yongqiang;Li, Jun
关键词：Tilapia; Hardness; Water holding capacity; Myofibrillar protein; Phosphorylation; Dissociation of actomyosin
