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Heparin-conjugated alginate multilayered microspheres for controlled release of bFGF

文献类型： 外文期刊

作者： Zuo, Qinhua 1 ; Guo, Rui 1 ; Liu, Quan 1 ; Hong, An 2 ; Shi, Yunfeng 1 ; Kong, Qian 3 ; Huang, Yuexin 4 ; He, Liumin 1 ; Xue 1 ;

作者机构： 1.Jinan Univ, Dept Biomed Engn, Key Lab Biomat Prot Res, Guangdong Higher Educ Inst, Guangzhou 510632, Guangdong, Peoples R China

2.Jinan Univ, Bioengn Inst, Coll Life Sci & Technol, Guangzhou 510632, Guangdong, Peoples R China

3.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangzhou 510640, Guangdong, Peoples R China

4.Guangdong Jianze Med Co Ltd, Guangzhou 510620, Guangdong, Pe

关键词： alginate microspheres;heparin;basic fibroblast growth factor (bFGF);layer-by-layer;controlled release

期刊名称：BIOMEDICAL MATERIALS （ 影响因子：3.715； 五年影响因子：3.987 ）

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收录情况： SCI

摘要： In order to effectively immobilize and control release of basic fibroblast growth factor (bFGF) from alginate microspheres, heparin-conjugated alginate (H-Alg) was first synthesized by covalent binding. Then multilayered H-Alg microspheres (multilayered microspheres) were fabricated via an electrostatic droplet generation technique followed by a layer-by-layer (LbL) self-assembly technique. Several techniques such as Fourier transform infrared spectroscopy (FTIR), H-1-NMR, zeta potential analysis and scanning electron microscopy (SEM) were used to characterize the properties of H-Alg (FTIR and H-1-NMR) and multilayered microspheres (FTIR, zeta potential analysis and SEM). bFGF binding efficiency, release profiles of bFGF from multilayered microspheres and the biological activity of released bFGF were well investigated. It was found that the bFGF binding efficiency of H-Alg microspheres was increased up to five times higher than that of the alginate microspheres. Additionally, the release profiles of bFGF from multilayered microspheres were sustained for two weeks with relieved initial burst release, and the release rate to bFGF could be regulated by controlling the number of deposited layers. Importantly, the released bFGF still retained its biological activity as assessed by the in vitro proliferation of NIH-3T3 mouse fibroblasts. In conclusion, this study presented an easy yet effective method for the controlled, sustained release of heparin-binding growth factors, using polyelectrolyte multilayer-coated heparin-conjugated alginate microspheres.

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