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Recombinant beta interferon could clear the low-dose infected porcine reproductive and respiratory syndrome virus (PRRSV) in MARC-145 cells

文献类型： 外文期刊

作者： Shi, X. 1 ; Zhang, X. 1 ; Wang, L. 3 ; Li, W. 1 ; Jiang, B. 4 ; Deng, R. 3 ; Wang, A. 5 ; Zhang, G. 2 ;

作者机构： 1.Henan Normal Univ, Coll Life Sci, Xinxiang 453000, Henan, Peoples R China

2.Henan Agr Univ, Coll Vet Med & Anim Sci, Zhengzhou 450002, Henan, Peoples R China

3.Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Key Lab Anim Immunol, Minist Agr, Zhengzhou 450002, Henan, Peoples R China

4.Chongqing Police Coll, Off Sci & Technol, Chongqing 401331, Peoples R China

5.Zhengzhou Univ, Dept Bioengn, Zhengzhou 450000, Peoples R China

关键词： PRRSV;recombinant IFN-beta

期刊名称：ACTA VIROLOGICA （ 影响因子：1.162； 五年影响因子：1.255 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Porcine reproductive and respiratory syndrome virus (PRRSV) causes one of the most economically devastating and pandemic porcine diseases. Previous study has shown that MARC-145 cells pretreated with recombinant IFN-beta (rIFN-beta) couldn't develop cytopathic effect (CPE) of PRRSV. However, up to date, it is not clear whether MARC-145 cells post-treated with rIFN-beta could develop CPE of PRRSV. The present work showed that the MARC-145 cells didn't develop the CPE at 120 hr post-infection (p.i.) with low-dose of PRRSV when the cells were pre-treated with rIFN-beta (Group 1), post-treated with rIFN-beta at 4 hr p.i. (Group 2), or post-treated with rIFN-beta at 8 hr p.i. (Group 3), while the MARC-145 cells could develop CPE when the cells were infected with high-dose PRRSV and then treated with rIFN-beta at 24 hr p.i.. Furthermore, the indirect immunofluorescence assay confirmed that there were a few N protein-positive cells in the high-dose infected cells in Group 1, Group 2 and Group 3, while there were no N protein-positive cells in the low-dose infected. cells in all rIFN-beta treatment groups. In addition, the numbers of N protein-positive cells in high-dose infected cells (MOI = 10) in Group 1 were lower than that in Group 2 and Group 3. The results above demonstrated that both pre-treatment with rIFN-beta and an earlier post-treatment with rIFN-beta could inhibit the PRRSV replication and could clear the low-dose infected PRRSV, which indicated that the rIFN-beta had efficient antiviral activities when the cells have been infected with PRRSV.

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[10]Small interfering RNA targeting nonstructural protein1 alpha (nsp1 alpha) of porcine reproductive and respiratory syndrome virus (PRRSV) can reduce the replication of PRRSV in MARC-145 cells. Shi, Xibao,Zhang, Xiaozhuan,Li, Chunxi,Shi, Xibao,Chang, Yongzhe,Zhang, Gaiping,Shi, Xibao,Chang, Yongzhe,Wang, Li,Chen, Jing,Wang, Chao,Deng, Ruiguang,Zhang, Gaiping,Zhang, Xiaozhuan,Jiang, Kai,Fan, Jianming.

[11]Nonstructural protein 11 (nsp11) of porcine reproductive and respiratory syndrome virus (PRRSV) promotes PRRSV infection in MARC-145 cells. Shi, Xibao,Zhang, Xiaozhuan,Shi, Xibao,Chang, Yongzhe,Deng, Ruiguang,Zhang, Gaiping,Zhang, Xiaozhuan,Zhang, Gaiping,Wang, Aiping,Jiang, Bo. 2016

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