文献类型： 外文期刊

作者： Zhang, H. ¹ ; Zhang, D. ¹ ; Han, S. ¹ ; Zhang, X. ¹ ; Yu, D. ¹ ;

作者机构： 1.Nanjing Agr Univ, Natl Ctr Soybean Improvement, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing, Peoples R China

2.Henan Acad Agr Sci, Econ Crops Res Inst, Zhengzhou, Peoples R China

关键词： soybean [Glycine max (L;) Merr;];chlorophyll;ethyl methanesulfonate;mutant;genetic mapping

期刊名称：PLANT BREEDING （ 影响因子：1.832； 五年影响因子：1.956 ）

ISSN： 0179-9541

年卷期： 2011 年 130 卷 2 期

页码：

收录情况： SCI

摘要： P>Chlorophyll variation mutants are important materials for studying chlorophyll metabolism, photosynthesis, chloroplast biogenesis and related physiological processes. In this study, a chlorophyll-deficient soybean mutant (cd1) was isolated by screening M-2 progenies of cultivar 'Nannong 86-4' treated with 0.4% ethyl methanesulfonate. cd1 displayed yellow-green leaves from seedling stage V1 to fully flowering stage R2, abnormal chloroplasts, lower total chlorophyll content (30-66% reduction), lower plant height and lower seed yield compared to its wild-type control. Genetic analysis demonstrated that the visible yellow-green trait was controlled by a single recessive nuclear gene. Subsequently, the cd1-mutant gene was mapped at a 6.9-cM interval on chromosome 10, flanked by two simple sequence repeat markers Satt633 and Sat_291. These results provide us with the opportunity to study a novel gene regulating chlorophyll synthesis.