文献类型： 外文期刊

作者： Zhao, R. ¹ ; Miao, H. ² ; Song, W. ¹ ; Chen, C. ¹ ; Zhang, H. ² ;

作者机构： 1.Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China

2.Henan Acad Agr Sci, Henan Sesame Res Ctr, Zhengzhou 450002, Henan, Peoples R China

关键词： bacterial artificial chromosome;chromosome;fluorescence insitu hybridisation;sesame (Sesamum indicum L;)

期刊名称：PLANT BIOLOGY （ 影响因子：3.081； 五年影响因子：2.972 ）

ISSN： 1435-8603

年卷期： 2018 年 20 卷 1 期

页码：

收录情况： SCI

摘要： Sesame (Sesamum indicum L.; Pedaliaceae) is a commercially valuable oilseed crop with high oil content. Its small genome size favours the genomic analysis of key biological processes, such as oil synthesis and metabolism. However, the 13 chromosome pairs of sesame have not been characterised because of technological limitations and their small size. We constructed a BAC library comprising 57,600 BAC clones for sesame. The estimated genome coverage of the sesame BAC library was 13.8x. The successive double colour fluorescence insitu hybridisation (FISH) with bacterial artificial chromosomes (BACs) for sesame was established in this study. Subsequently, the 13 sesame chromosome pairs were individually differentiated using 17 specific BACs for the first time. The schematic of the sesame chromosome set was drawn according to the chromosome relative length and relative position of the BAC signal. The cytogenetic characteristics of sesame chromosomes were also explored. The results provide the technical background required for further cytogenetic map construction, genome assembly and localisation of the DNA sequence in sesame.