Efficient biosynthesis of (2S)-pinocembrin from D-glucose by integrating engineering central metabolic pathways with a pH-shift control strategy
文献类型： 外文期刊
作者： Wu, Junjun 1 ; Zhang, Xia 1 ; Zhou, Jingwen 2 ; Dong, Mingsheng 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Food Sci & Technol, 1 Weigang Rd, Nanjing 210095, Jiangsu, Peoples R China
2.Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Ind Biotechnol, 1800 Lihu Rd, Wuxi 214122, Jiangsu, Peoples R China
3.Jiangsu Acad Agr Sci, Inst Agroprod Proc, Nanjing 210095, Jiangsu, Peoples R China
关键词： Microorganisms;CRISPRi;Gene repressing;Plant natural products;Low-cost production
期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：9.642； 五年影响因子：9.237 ）
ISSN：
年卷期：
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收录情况： SCI
摘要： Microbial fermentations promise to revolutionize the conventional extraction of (2S)-pinocembrin from natural plant sources. Previously an Escherichia coli fermentation system was developed for one-step (2S)-pinocembrin production. However, this fermentation platform need supplementation of expensive malonyl-CoA precursor malonate and requires morpholinopropane sulfonate to provide buffering capacity. Here, a clustered regularly interspaced short palindromic repeats interference was constructed to efficiently channel carbon flux toward malonyl-CoA. By exploring the effects of different culture pH on microbial fermentation, it was found that high pH values favored upstream pathway catalysis, while low pH values favored downstream pathway catalysis. Based on this theory, a two-stage pH control strategy was proposed. The pH was controlled at 7.0 during 0-10 h, and was shifted to 6.5 after 10 h. Finally, the (2S)-pinocembrin titers increased to 525.8 mg/L. These results were attained in minimal medium without additional precursor supplementation, thus offering opportunities for industrial scale low-cost production of flavonoids. (C) 2016 Elsevier Ltd. All rights reserved.
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