您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Molecular cloning of the rabbit interleukin 6 promoter: Functional characterization of rabbit hemorrhagic disease virus response elements in RK-13 cells

文献类型： 外文期刊

作者： Liu, Xing 1 ; Hu, Bo 1 ; Wang, Fang 1 ; Song, Yanhua 1 ; Fan, Zhiyu 1 ; Wei, Houjun 1 ; Qiu, Rulong 1 ; Xu, Weizhong 2 ;

作者机构： 1.Jiangsu Acad Agr Sci, Natl Ctr Engn Res Vet Bioprod, Key Lab Vet Biol Engn & Technol, Inst Vet Med,Minist Agr, Nanjing 210014, Peoples R China

2.Jiangsu Acad Agr

关键词： RHDV;IL-6 promoter;AP-1;NF-IL6;NF-kappa B

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Infection with rabbit hemorrhagic disease virus (RHDV) can cause acute liver failure (ALF), leading to severe mortality in rabbits, Inflammatory response, especially the expression of inflammatory cytokines such as interleulcin (IL)-1 beta, tumor necrosis factor (TNF)-alpha, and IL-6, may play major roles in mediating and amplifying the ALF. Among these cytokines, IL-6 is a multifunctional cytokine with a central role in various physiological inflammatory and immunological processes. In this study, we found that RHDV infection significantly upregulated IL-6 gene expression in vivo. Next, the rabbit IL-6 promoter was cloned and analyzed. Transfection of full-length RHDV cDNA in RK-13 cells upregulated the activity of the IL-6 promoter. A series of 5' deletion constructs demonstrated that AP-1 (activator protein 1), NF-IL6 (nuclear factor interleukin-6), and NF-kappa B (nuclear factor kappa B) elements were critical for RHDV-induced IL-6 transcription. Besides, the CREB (cAMP-response element binding protein) element may also play an accessory effect on RHDV-induced IL-6 transcription. Collectively, the results elucidate the mechanism of IL-6 induction, and enrich the RHDV pathogenesis in rabbit. (C) 2016 Elsevier Ltd. All rights reserved.

  • 相关文献

[1]RHDV在多种属动物体内分布及细胞免疫水平. 金明兰,侯继波,郑其升,鲁会军,刘春霞,南文龙,任静强,韩松,金宁一. 2013

[2]几种提纯RHDV方法的比较研究. 杨龙圣,薛家宾,丁国明,徐为中,陈兴祥,孔祥峰,胡元亮. 2006

[3]嵌合双串联OVA T细胞表位的RHDV VP60蛋白免疫特性研究. 宋艳华,张燕,胡波,范志宇,魏后军,刘星,黄兵,薛家宾,徐为中. 2015

[4]Recombination between G2 and G6 strains of rabbit hemorrhagic disease virus (RHDV) in China. Hu, Bo,Wang, Fang,Fan, Zhiyu,Song, Yanhua,Zuo, Yuanyuan,Hu, Bo,Wang, Fang,Fan, Zhiyu,Song, Yanhua,Zuo, Yuanyuan,Abrantes, Joana,Esteves, Pedro J.,Esteves, Pedro J.,Esteves, Pedro J..

[5]A new variant of rabbit hemorrhagic disease virus G2-like strain isolated in China. Hu, Bo,Fan, Zhiyu,Wang, Fang,Song, Yanhua,Wei, Houjun,Liu, Xing,Qiu, Rulong,Xu, Weizhong,Xue, Jiabin,Hu, Bo,Fan, Zhiyu,Wang, Fang,Song, Yanhua,Wei, Houjun,Liu, Xing,Qiu, Rulong,Xu, Weizhong,Xue, Jiabin,Yuan, Wanzhe.

[6]NF-kappa B target microRNAs and their target genes in TNF alpha-stimulated HeLa Cells. Zhou, Fei,Wang, Wei,Xing, Yujun,Wang, Tingting,Xu, Xinhui,Wang, Jinke,Xing, Yujun. 2014

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台