Molecular cloning of the rabbit interleukin 6 promoter: Functional characterization of rabbit hemorrhagic disease virus response elements in RK-13 cells
文献类型： 外文期刊
作者： Liu, Xing 1 ; Hu, Bo 1 ; Wang, Fang 1 ; Song, Yanhua 1 ; Fan, Zhiyu 1 ; Wei, Houjun 1 ; Qiu, Rulong 1 ; Xu, Weizhong 2 ;
作者机构： 1.Jiangsu Acad Agr Sci, Natl Ctr Engn Res Vet Bioprod, Key Lab Vet Biol Engn & Technol, Inst Vet Med,Minist Agr, Nanjing 210014, Peoples R China
2.Jiangsu Acad Agr
关键词： RHDV;IL-6 promoter;AP-1;NF-IL6;NF-kappa B
期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： Infection with rabbit hemorrhagic disease virus (RHDV) can cause acute liver failure (ALF), leading to severe mortality in rabbits, Inflammatory response, especially the expression of inflammatory cytokines such as interleulcin (IL)-1 beta, tumor necrosis factor (TNF)-alpha, and IL-6, may play major roles in mediating and amplifying the ALF. Among these cytokines, IL-6 is a multifunctional cytokine with a central role in various physiological inflammatory and immunological processes. In this study, we found that RHDV infection significantly upregulated IL-6 gene expression in vivo. Next, the rabbit IL-6 promoter was cloned and analyzed. Transfection of full-length RHDV cDNA in RK-13 cells upregulated the activity of the IL-6 promoter. A series of 5' deletion constructs demonstrated that AP-1 (activator protein 1), NF-IL6 (nuclear factor interleukin-6), and NF-kappa B (nuclear factor kappa B) elements were critical for RHDV-induced IL-6 transcription. Besides, the CREB (cAMP-response element binding protein) element may also play an accessory effect on RHDV-induced IL-6 transcription. Collectively, the results elucidate the mechanism of IL-6 induction, and enrich the RHDV pathogenesis in rabbit. (C) 2016 Elsevier Ltd. All rights reserved.
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