Transcriptome and digital gene expression analysis of herbaceous peony (Paeonia lactiflora Pall.) to screen thermo-tolerant related differently expressed genes
文献类型: 外文期刊
作者: Hao, Zhaojun 1 ; Wei, Mengran 2 ; Gong, Saijie 2 ; Zhao, Daqiu 2 ; Tao, Jun 1 ;
作者机构: 1.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Jiangsu, Peoples R China
2.Yangzhou Univ, Coll Hort & Plant Protect, Key Lab Crop Genet & Physiol, Yangzhou 225009, Jiangsu, Peoples R China
3.Jiangsu Key Lab Hort Crop Genet Improvement, Nanjing 210014, Jiangsu, Peoples R China
关键词: Transcriptome;RNA-seq;Heat stress;Herbaceous peony
期刊名称:GENES & GENOMICS ( 影响因子:1.839; 五年影响因子:1.329 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Herbaceous peony (Paeonia lactiflora Pall.) is easily injured by heat stress (HS), which greatly restricts its application and promotion. In this study, the thermo-tolerance of three representative P. lactiflora cultivars had been firstly assessed. 'Zifengyu' was identified as the thermo-tolerant cultivar with relatively lower values and smaller variations in malondialdehyde, hydrogen peroxide (H2O2) and proline contents under HS. Subsequently, their transcriptomes were sequenced by RNA sequencing (RNA-seq) technology to construct a complete database. 81,599 unigenes were obtained, and 34,940 unigenes had been annotated. Moreover, through digital gene expression analysis of thermo-tolerant 'Zifengyu' and moderately thermo-tolerant 'Hongyanzhenghui', 161 heat stress response genes had been screened involving heat shock protein genes, plant hormone signal transduction related genes, fatty acid synthesis genes, reactive oxygen species-scavenging genes and secondary metabolites related genes. And the effectively and timely response of these genes to HS could endow thermo-tolerance to 'Zifengyu'. Among these genes, 11 key thermo-tolerant related genes whose expressions were all significantly up-regulated in 'Zifengyu' and 'Hongyanzhenghui' during development and the former possessed higher levels could be regarded as the candidate genes, including isoprene synthase gene, 2 peroxidase genes, 3-oxoacyl-acyl carrier protein reductase gene (FabG), 3 transcription factor genes (bHLH, NAC and WRKY), HSP20 and 3 HSP70. These results could provide a better understanding of heat stress response in P. lactiflora, and pave for the breeding of thermo-tolerant cultivars.
- 相关文献
作者其他论文 更多>>
-
Deterioration of orthodox seeds during ageing: Influencing factors, physiological alterations and the role of reactive oxygen species
作者:Zhang, Keliang;Zhang, Yin;Sun, Jing;Meng, Jiasong;Tao, Jun
关键词:Seed ageing; Reactive oxygen species; Lipid peroxidation; Oxidative stress; Protein carbonylation; Programmed cell death
-
Dormancy-breaking and germination requirements of Thalictrum squarrosum Stephan ex Willd. seeds with underdeveloped embryos
作者:Zhang, Keliang;Ji, Yusong;Fu, Guixian;Yao, Linjun;Liu, Huina;Tao, Jun
关键词:Morphophysiological dormancy; Seed germination; Stratification; Thalictrum squarrosum; Underdeveloped embryos
-
y Deep complex morphophysiological dormancy in seeds of Clematis hexapetala Pall. (Ranunculaceae)
作者:Zhang, Keliang;Ji, Yusong;Song, Xiaomei;Yao, Linjun;Liu, Huina;Tao, Jun
关键词:Clematis hexapetalai; Underdeveloped embryos; Cold stratification; Morphophysiological dormancy; Seed germination; Ranunculaceae
-
Biochemical and molecular responses of herbaceous peony to high temperature stress
作者:Wu, Yan-Qing;Zhao, Da-Qiu;Han, Chen-Xia;Tao, Jun;Zhao, Da-Qiu;Tao, Jun
关键词:herbaceous peony;high temperature stress;physiological mechanism;heat shock protein
-
Shade Ameliorates High Temperature-induced Inhibition of Growth in Herbaceous Peony (Paeonia lactiflora)
作者:Zhao, Daqiu;Han, Chengxia;Zhou, Chunhua;Tao, Jun;Zhao, Daqiu
关键词:Herbaceous peony;High temperature stress;Heat shock protein;Thermal damage
-
Comparative Analysis of Headspace Volatiles of Chinese Rosa rugosa
作者:Liu, Ping;Zhao, Lan-Yong;Feng, Li-Guo;Chen, Chen;Sheng, Li-Xia;Tao, Jun;Su, Jia-Le
关键词:Rosa rugosa;headspace volatiles;content;gas chromatography-mass spectrometry
-
Anatomical and biochemical analysis reveal the role of anthocyanins in flower coloration of herbaceous peony
作者:Zhao, Da-Qiu;Wei, Meng-Ran;Liu, Ding;Tao, Jun;Zhao, Da-Qiu;Wei, Meng-Ran;Liu, Ding;Tao, Jun
关键词:Herbaceous peony;Anthocyanins;Red colour;Anatomical analysis