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Identification of heat shock protein A9 as a Tembusu virus binding protein on DF-1 cells

文献类型： 外文期刊

作者： Liu, Qingtao 1 ; Huang, Xinmei 1 ; Zhao, Dongmin 1 ; Han, Kaikai 1 ; Liu, Yuzhuo 1 ; Yang, Jing 1 ; Bi, Keran 1 ; Li, Yin 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Vet Med, Key Lab Vet Biol Engn & Technol, Minist Agr, Nanjing, Jiangsu, Peoples R China

关键词： Tembusu virus;Heat shock protein A9;Receptor

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

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收录情况： SCI

摘要： This study attempts to identify receptor elements for Tembusu virus (TMUV) on DF-1 cells. Using coimmunoprecipitation and virus overlay protein binding assays, we identified a TMUV-binding protein of approximately 70-kDa on DF-1 cell membranes. Mass spectroscopy identified the protein to be heat shock protein (HSP) A9, which was reconfirmed by an anti-HSPA9 antibody. Indirect immunofluorescence demonstrated a significant degree of colocalization between HSPA9 and TMUV on cell surface. Additionally, an antibody against HSPA9 could inhibit TMUV infection in DF-1 cells in a dose-dependent manner. These results might suggest that HSPA9 is a putative receptor for TMUV. (C) 2016 Elsevier B.V. All rights reserved.

  • 相关文献

[1]Design and evaluation of a polytope construct with multiple B and T epitopes against Tembusu virus infection in ducks. Han, Kaikai,Zhao, Dongmin,Liu, Yuzhuo,Huang, Xinmei,Yang, Jing,Liu, Qingtao,An, Fengjiao,Li, Yin.

[2]Identification and molecular characterization of a novel flavivirus isolated from geese in China. Huang, Xinmei,Han, Kaikai,Zhao, Dongmin,Liu, Yuzhuo,Zhang, Jingfeng,Niu, Huimin,Li, Yin,Zhang, Kangning,Zhu, Jiangning,Wu, Dongming,Gao, Lei.

[3]Quantitative Proteomic Analysis of Duck Ovarian Follicles Infected with Duck Tembusu Virus by Label-Free LC-MS. Han, Kaikai,Zhao, Dongmin,Liu, Yuzhuo,Liu, Qingtao,Huang, Xinmei,Yang, Jing,An, Fengjiao,Lin, Yin,Han, Kaikai,Zhao, Dongmin,Liu, Yuzhuo,Liu, Qingtao,Huang, Xinmei,Yang, Jing,An, Fengjiao,Lin, Yin. 2016

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