文献类型： 外文期刊

作者： Niu, Lili ¹ ; Xiong, Mengjie ¹ ; Zhu, Daochen ² ; Song, Lei ³ ; Tang, Tianyi ¹ ; Li, Wenying ⁴ ;

作者机构： 1.Shanghai Univ, Sch Life Sci, Shanghai Key Lab Bioenergy Crops, Shanghai 200444, Peoples R China

2.Jiangsu Univ, Sch Environm Engn, Zhenjiang 212013, Peoples R China

3.Chinese Acad Sci, China Gen Microbiol Culture Collect Ctr, Inst Microbiol, Beijing 100101, Peoples R China

4.Guangdong Acad Agr Sci, Inst Agr Resources & Environm, Guangzhou 510640, Guangdong, Peoples R China

期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.747； 五年影响因子：2.889 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A novel Gram-stain-positive, strictly aerobic, endospore-forming, rod-shaped bacterial strain 7578-24(T) was isolated from ripened Pu'er tea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 7578-24(T) clustered with species of the genus Pullulanibacillus in the family Sporolactobacillaceae with 97.8-95.2% sequence similarities, and was most closely related to Pullulanibacillus pueri YN3T with 97.8% 16S rRNA gene sequence similarity. The DNA-DNA relatedness value between strain 7578-24(T) and P. pueri YN3(T) was 35 %. Strain 7578-24(T) had a cell-wall type A1 gamma peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid. The major menaquinone was menaquinone 7 (MK-7). C-18 : 1 omega 7c (45.4 %), anteiso-C-17 : 0 (30.6 %) and anteiso-C-15 : 0 (10.1 %) were the predominant fatty acids, and diphosphatidylglycerol, phosphatidylglycerol, five unknown phospholipids and one unknown aminolipid were the major polar lipids. The DNA G+C content of strain 7578-24(T) was 45.2 mol %. Strain 7578-24(T) could be differentiated from other related species of the genus Pullulanibacillus based on phenotypic characteristics, chemotaxonomic differences, phylogenetic analysis and DNA-DNA hybridization data. On the basis of polyphasic evidence from this study, a novel species of the genus Pullulanibacillus named Pullulanibacillus camelliae sp. nov. is proposed, with strain 7578-24(T) (=CGMCC 1.15371(T) =JCM 31236(T)) as the type strain.