文献类型： 外文期刊

作者： Niu, Lili ¹ ; Xiong, Mengjie ¹ ; Zhang, Juan ¹ ; Xiang, Yangquan ¹ ; Song, Lei ⁴ ; Hua, Ziyi ⁵ ; Li, Wenying ⁶ ;

作者机构： 1.Shanghai Univ, Sch Life Sci, Shanghai Key Lab Bioenergy Crops, Shanghai 200444, Peoples R China

2.Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China

3.Sun Yat Sen Univ, Sch Life Sci, Guangdong Prov Key Lab Plant Resources, Guangzhou 510275, Guangdong, Peoples R China

4.Chinese Acad Sci, Inst Microbiol, China Gen Microbiol Culture Collect Ctr, Beijing 100101, Peoples R China

5.Shanghai Univ, Sch Life Sci, Expt Ctr Life Sci, Shanghai 200444, Peoples R China

6.Guangdong Acad Agr Sci, Inst Agr Resources & Environm, Guangzhou 510640, Guangdong, Peoples R China

关键词： Bacillus camelliae sp nov.;polyphasic analysis;Pu'er tea

期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.747； 五年影响因子：2.889 ）

ISSN： 1466-5026

年卷期： 2018 年 68 卷 2 期

页码：

收录情况： SCI

摘要： A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain, 7578-1(T), was isolated from ripened Pu'er tea. Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1(T) was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletonii LMG 18435(T) (98.4 %), Bacillus acidicola DSM 14745(T) (97.6 %), Bacillus paralicheniformis KACC 18426(T) (97.2 %) and Bacillus ginsengihumi KCTC 13944(T) (96.7 %). The fatty acid profile containing the major fatty acids, iso-C-15 : 0, anteiso-C-15 : 0 and anteiso-C-17 : 0 supported the allocation of strain 7578-1(T) to the genus Bacillus. The strain had a cell-wall type A1 gamma peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid. The major menaquinone was MK-7 (95 %). The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, one unidentified phospholipid and one unidentified lipid. The average nucleotide identity values between strain 7578-1(T) and its most closely related species were 67.8-82.4% by OrthoANIu analysis. The DNA-DNA relatedness value between strain 7578-1(T) and the type strains of closely related species were 17-39 %, again indicating that strain 7578-1(T) represented a novel species in the genus Bacillus. The DNA G+C content of strain 7578-1(T) was 36.0 mol%. On the basis of the presented polyphasic evidence, strain 7578-1(T) is considered to represent a novel species of the genus Bacillus, for which we propose the name Bacillus camelliae sp. nov. The type strain is 7578-1(T) (=CGMCC 1.15374(T) =KCTC 33845(T)).