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Multiplexed immunochromatographic test strip for time-resolved chemiluminescent detection of pesticide residues using a bifunctional antibody

文献类型： 外文期刊

作者： Shu, Qi 1 ; Wang, Limin 2 ; Ouyang, Hui 1 ; Wang, Wenwen 1 ; Liu, Fengquan 2 ; Fu, Zhifeng 1 ;

作者机构： 1.Southwest Univ, Coll Pharmaceut Sci, Minist Educ, Key Lab Luminescence & Real Time Analyt Chem, Chongqing 400716, Peoples R China

2.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China

关键词： Methyl parathion;Imidacloprid;Bifunctional antibody;Time-resolved chemiluminescence;Immunochromatographic test strip;Multiplexed immunoassay

期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：10.618； 五年影响因子：9.323 ）

ISSN：

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收录情况： SCI

摘要： A novel bifunctional antibody (BfAb) that could recognize methyl parathion and imidacloprid simultaneously was prepared by a hybrid hybridomas technique. Using the BfAb as the sole recognition reagent, a multiplexed immunochromatographic test strip based on a time-resolved chemiluminescence (CL) strategy was developed for quantitative detection of pesticide residues. Horseradish peroxidase (HRP) and alkaline phosphatase (ALP) were used as the CL probes to label the haptens of methyl parathion and imidacloprid, respectively. After the labeled haptens competed with methyl parathion and imidacloprid to bind with the BfAb immobilized on the test strip, the two CL reactions catalyzed by the enzymes were triggered simultaneously by coreactants injection. Due to the distinct CL kinetics characteristics of HRP and ALP, the signals for methyl parathion and imidacloprid detections were collected at 2.5 s and 300 s, respectively. The linear ranges for methyl parathion and imidacloprid were both 0.1-250 ng mL(-1), with detection limits of 0.058 ng mL(-1) (S/N=3). The whole assay process could be accomplished within 22 min. The detection results for spiked traditional Chinese medicine samples demonstrated its application potential. The proposed method provided a low-cost, facile and rapid tool for multiplexed screening of pesticide residues using single antibody.

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[1]Simultaneous detection of dual biomarkers from humans exposed to organophosphorus pesticides by combination of immunochromatographic test strip and ellman assay. Yang, Mingming,Zhao, Yuting,Du, Dan,Lin, Yuehe,Yang, Mingming,Liu, Fengquan,Paulsen, Michael,Simpson, Christopher D.,Wang, Limin. 2018

[2]STATUS OF INSECTICIDE RESISTANCE OF THE WHITEBACKED PLANTHOPPER, SOGATELLA FURCIFERA (HEMIPTERA: DELPHACIDAE). Su, Jianya,Wang, Zhiwei,Zhang, Kai,Tian, Xiangrui,Gao, Cong Fen,Yin, Yanqiong,Zhao, Xueqing,Shen, Aidong. 2013

[3]A bare-eye based one-step signal amplified semiquantitative immunochromatographic assay for the detection of imidacloprid in Chinese cabbage samples. Fang, Qingkui,Wang, Limin,Cheng, Qi,Cai, Jia,Wang, Yulong,Yang, Mingming,Hua, Xiude,Liu, Fengquan,Liu, Fengquan.

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