Development and application of an in-cell cleanup pressurized liquid extraction with ultra-high-performance liquid chromatography-tandem mass spectrometry to detect prohibited antiviral agents sensitively in livestock and poultry feces
文献类型： 外文期刊
作者： Wu, Huizhen 1 ; Wang, Jianmei 2 ; Yang, Hua 2 ; Li, Guoqin 3 ; Zeng, Yinhuan 2 ; Xia, Wei 1 ; Li, Zuguang 1 ; Qian, Mingr 1 ;
作者机构： 1.Zhejiang Univ Technol, Coll Chem Engn, Hangzhou 310014, Zhejiang, Peoples R China
2.Zhejiang Acad Agr Sci, Inst Qual & Standard Agroprod, MOA Key Lab Pesticide Residue Detect, Hangzhou 310021, Zhejiang, Peoples R China
3.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Sci, Hangzhou 310021, Zhejiang, Peoples R China
关键词： Antiviral agents;Pressurized liquid extraction;Livestock and poultry feces
期刊名称：JOURNAL OF CHROMATOGRAPHY A （ 影响因子：4.759； 五年影响因子：4.302 ）
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收录情况： SCI
摘要： An in-cell cleanup pressurized liquid extraction was developed to analyze prohibited antiviral agents in livestock and poultry feces. Extraction and cleanup were integrated into one step. The extraction was performed using methanol-acetonitrile (1:1, v/v) with 0.5% glacial acetic acid at 90 degrees C, and 0.75 g of PSA was used as the adsorbent during the extraction procedure. Under optimal conditions, the average recoveries for 11 antiviral drugs were 71.5-112.5% at three spiked levels (20, 40, and 100 mu g kg(-1)). The detection limits and detection quantitations of the analysis method for the eleven antiviral drugs were 0.6-1.4 and 1.4-4.7 mu g kg(-1), respectively. Finally, the method was applied to analyze amantadine, oseltamivir and its metabolites oseltamivir acid in duck feces based on an experiment of an oral dose of two antiviral drugs in duck. The amantadine, oseltamivir and oseltamivir acid can be detected in feces within approximately four weeks after amantadine and oseltamivir were orally administered. The results indicate that the residue analysis in feces is a noninvasive method to monitor inhibited antiviral agents efficiently in livestock and poultry breeding. (C) 2017 Published by Elsevier B.V.
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