Aptamer-molecularly imprinted sensor base on electrogenerated chemiluminescence energy transfer for detection of lincomycin
文献类型： 外文期刊
作者： Li, Shuhuai 1 ; Liu, Chunhua 1 ; Yin, Guihao 1 ; Zhang, Qun 1 ; Luo, Jinhui 1 ; Wu, Nanchun 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Anal & Test Ctr, Haikou 571101, Peoples R China; Haikou Minist Agr, Lab Qual & Safety Risk Assessment Trop Prod, Haikou 571101, Peoples R China
关键词： Dual recognition;Aptamer;Molecularly imprinted;lincomycin;Energy transfer
期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：10.618； 五年影响因子：9.323 ）
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收录情况： SCI
摘要： In this study, a biosensor with a dual recognition system comprising a molecularly imprinted polymer (MIP) and aptamers selective for lincomycin was fabricated. The MIP was synthesized by electropolymerization of carbon dots (C-dots)-tagged DNA aptamers combined with lincomycin and o-aminophenol on the goldnanoparticle-functionalized graphene oxide (Au-GO)-modified electrode. Electrogenerated chemiluminescence (ECL) resonance energy transfer was observed between Au-GO and C-dots. After the C-dots accepted the energy, they acted as a signal indicator and exhibited enhanced signal intensity in the presence of target lincomycin. When lincomycin was competitively bound to DNA aptamers and MIP, it blocked the transfer of energy, and a decreased ECL signal was observed. Hence, a dual recognition method for the detection of lincomycin is realized. Using this strategy, the sensor exhibited a linear ECL response to lincomycin at concentrations from 5.0 x 10(-12) mol/L to 1.0 x 10(-9) mol/L. The detection limit of this assay was found to be 1.6 x 10(-13) mol/L. This method was utilized to determine lincomycin residuals in meat samples with satisfactory results.
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