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Optimization of Conditions for Cyanidin-3-O-Glucoside (C3G) Nanoliposome Production by Response Surface Methodology and Cellular Uptake Studies in Caco-2 Cells

文献类型： 外文期刊

作者： Liang, Tisong 1 ; Guan, Rongfa 1 ; Shen, Haitao 2 ; Xia, Qile 3 ; Liu, Mingqi 1 ;

作者机构： 1.China Jiliang Univ, Zhejiang Prov Key Lab Biometrol & Inspect & Quara, Hangzhou 310018, Zhejiang, Peoples R China

2.Zhejiang Prov Ctr Dis Control & Prevent, Hangzhou 310051, Zhejiang, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Food Sci, 298 Desheng Rd, Hangzhou 310021, Zhejiang, Peoples R China

关键词： C3G;nanoliposomes;response surface methodology;stability;cell viability

期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： We aimed to optimize the formulation of C3G nanoliposomes using response surface methodology. Additionally, we evaluated the stability, particle change, and encapsulation efficiency (EE) of C3G nanoliposomes under different temperatures and storage durations, as well as in simulated gastrointestinal juice (SGF) and simulated intestinal fluid. The morphology of C3G nanoliposomes was observed by transmission electron microscope. The ability of C3G nanoliposomes to affect cancer cell morphology and inhibit cancer cell proliferation was studied with Caco-2 cells. Reverse-phase evaporation method is a simple and efficient method for liposome preparation. The optimal preparation conditions for this method were as follows: C3G concentration of 0.17 mg/mL, phosphatidylcholine/cholesterol ratio of 2.87, and rotary evaporation temperature of 41.41 degrees C. At optimal conditions, the particle size and EE of the C3G nanoliposomes were 165.78 +/- 4.3 nm and 70.43% +/- 1.95%, respectively. The C3G nanoliposomes showed an acceptable stability in SGF at 37 degrees C for 4 h, but were unstable under extended storage durations and high temperatures. Moreover, our results showed that different concentrations of C3G nanoliposomes affected the morphology and inhibited the proliferation of Caco-2 cells.

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