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The Siderophore Product Ornibactin Is Required for the Bactericidal Activity of Burkholderia contaminans MS14

文献类型： 外文期刊

作者： Deng, Peng 1 ; Foxfire, Adam 2 ; Xu, Jianhong 1 ; Baird, Sonya M. 1 ; Jia, Jiayuan 1 ; Delgado, Keren H. 2 ; Shin, Ron 1 ;

作者机构： 1.Mississippi State Univ, Dept Biochem Mol Biol Entomol & Plant Pathol, Mississippi State, MS 39762 USA

2.Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA

3.Jiangsu Acad Agr Sci, Key Lab Food Qual & Safety Jiangsu Prov, State Key Lab Breeding Base, Nanjing, Jiangsu, Peoples R China

4.Univ Alabama Birmingham, Ctr Canc, Struct Biol Shared Facil, Cent Alabama High Field NMR Facil, Birmingham, AL USA

5.Cornell Univ, Weill Cornell Med, New York, NY 10021 USA

关键词： Burkholderia contaminans MS14;antibacterial activity;ornibactin

期刊名称：APPLIED AND ENVIRONMENTAL MICROBIOLOGY （ 影响因子：4.792； 五年影响因子：5.26 ）

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收录情况： SCI

摘要： Burkholderia contaminans MS14 was isolated from soil in Mississippi. When it is cultivated on nutrient broth-yeast extract agar, the colonies exhibit bactericidal activity against a wide range of plant-pathogenic bacteria. A bacteriostatic compound with siderophore activity was successfully purified and was determined by nuclear magnetic resonance spectroscopy to be ornibactin. Isolation of the bactericidal compound has not yet been achieved; therefore, the exact nature of the bactericidal compound is still unknown. During an attempt to isolate the bactericidal compound, an interesting relationship between the production of ornibactin and the bactericidal activity of MS14 was characterized. Transposon mutagenesis resulted in two strains that lost bactericidal activity, with insertional mutations in a nonribosomal peptide synthetase (NRPS) gene for ornibactin biosynthesis and a luxR family transcriptional regulatory gene. Coculture of these two mutant strains resulted in restoration of the bactericidal activity. Furthermore, the addition of ornibactin to the NRPS mutant restored the bactericidal phenotype. It has been demonstrated that, in MS14, ornibactin has an alternative function, aside from iron sequestration. Comparison of the ornibactin biosynthesis genes in Burkholderia species shows diversity among the regulatory elements, while the gene products for ornibactin synthesis are conserved. This is an interesting observation, given that ornibactin is thought to have the same defined function within Burkholderia species. Ornibactin is produced by most Burkholderia species, and its role in regulating the production of secondary metabolites should be investigated.

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